ZFIN ID: ZDB-FIG-151110-32
Radev et al., 2015 - A TALEN-Exon Skipping Design for a Bethlem Myopathy Model in Zebrafish. PLoS One   10:e0133986 Full text @ PLoS One
Anatomical Terms:
Stage Range: Long-pec to Adult

Fig. 4

Validation of the presence of col6a1 mRNA by RT-PCR at different developmental stages in different organs of zebrafish.

(A) Confirmation of the presence of col6a1 mRNA in different organs of adult WT zebrafish: col6a1 mRNA is detected in the electrophoregram of organs from adult fish as indicated at the top of the panel. β-actin is used as internal control. (B) Confirmation of the presence of two forms of col6a1 mRNA in wild-type (WT) and mutant (presenting exon 14 skipping) zebrafish embryos at 2 dpf from col6a1ama605003 line. WT fish express only the wild type allele (298 bp); heterozyogous mutant (HT) carry both wild type and mutated allele (244 bp) and homozygous fish (HM) express only the exon-skipped form.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col6a1 TU standard conditions Long-pec anatomical structure ISH
Adult caudal fin ISH
Adult intestine ISH
Adult mandibular arch skeleton ISH
Adult muscle ISH
Adult spleen ISH
Antibody Labeling Details No data available
Phenotype Details No data available
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