ZFIN ID: ZDB-FIG-151009-53
Li-Villarreal et al., 2015 - Dachsous1b cadherin regulates actin and microtubule cytoskeleton during early zebrafish embryogenesis. Development (Cambridge, England)   142(15):2704-18 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 1-cell to 30%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage Range: 1-cell to 4-cell

Fig. 7

Abnormal wnt8 expression-domain shift and vegetal microtubule populations in MZdchs1b mutants, and dchs1b RNA rescue of the microtubule phenotype. (A) wnt8a expression in WT and MZdchs1b embryos at four- and 32-cell stages. Black bars mark the angle between the edge of wnt8a expression domain from the vegetal pole. (B) Quantification of the angle between the edge of the wnt8a expression domain and the vegetal pole for WT and MZdchs1b embryos at four- and 32-cell stages. ***P<0.005. (C) DM1α labeling of vegetal-pole microtubules for WT and MZdchs1b embryos at 20mpa. Top panels show parallel microtubule arrays in tangential view of embryos. Bottom panels show higher magnification views, with black arrowheads indicating crossing microtubules. (D) Plot profile of WT and MZdchs1b parallel arrays for intensity. (E) DM1α labeling of microtubules in WT, MZdchs1b and MZdchs1b embryos injected with dchs1b full-length RNA and ICD RNA at 40% epiboly. (F) Quantification of rescue-of-yolk microtubule phenotype, with different doses of dchs1b full-length or ICD RNAs. MZdchs1b is statistically different from all other conditions. ns, not significant; ***P<0.0001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
wnt8a AB standard conditions 4-cell blastomere ISH
32-cell blastomere ISH
4-cell yolk ISH
32-cell yolk ISH
dchs1bfh275/fh275 standard conditions 32-cell blastomere ISH
4-cell blastomere ISH
32-cell yolk ISH
4-cell yolk ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-tuba IHC AB standard conditions 1-cell fertilized egg microtubule
IHC 30%-epiboly whole organism microtubule
IHC dchs1bfh275/fh275 standard conditions 1-cell fertilized egg microtubule
IHC 30%-epiboly whole organism microtubule
Phenotype Details
Fish Conditions Stage Phenotype
dchs1bfh275/fh275 standard conditions 1-cell fertilized egg microtubule disoriented, abnormal
1-cell fertilized egg microtubule irregular spatial pattern, abnormal
4-cell yolk microtubule disoriented, abnormal
4-cell yolk microtubule irregular spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development