Fig. S3

Vangl2 protein expression from 30% epiboly to 80% epiboly stages. Confocal images of embryos after immunostaining using the anti-C-terminal zVangl2 antibody (green) and anti-β-catenin (red). β-catenin staining was used as control in order to visualize the cell membranes. Several embryos are shown in order to represent the slight variability of the anti-Vangl2 staining at the membrane versus cytoplasm between embryos at the same developmental stage. (A-C′) 3 different embryos at 30% epiboly stage (4.7hpf). Images were acquired at the animal pole location. (D-F′) 3 different embryos at germ ring stage (5.7hpf) (images acquired at the animal pole location). (G-G′) embryos at germ ring stage (images acquired at the embryonic margin location). (H-H′) embryos at 80% epiboly stage (8.4hpf) (images acquired at the dorsal location). Scale bars, 20 µm.