ZFIN Figure: Yan et al., 2015, Fig. 3

ZFIN ID: ZDB-FIG-150923-10

ZFIN ID: ZDB-FIG-150923-10

Yan et al., 2015 - Stimulation of hepatocarcinogenesis by neutrophils upon induction of oncogenic kras expression in transgenic zebrafish. Journal of hepatology 63(2):420-8 Full text @ J. Hepatol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	DsRed2 EGFP
Fish:	nz50Tg nz50Tg + MO1-irf8 nz50Tg + MO3-csf3r gz32Tg; nz50Tg gz32Tg; nz50Tg + MO1-irf8 gz32Tg; nz50Tg + MO3-csf3r
Condition:	chemical treatment: doxycycline monohydrate
Knockdown Reagents:	MO1-irf8 MO3-csf3r
Anatomical Terms:	liver neutrophil
Stage:	Day 6

PHENOTYPE:

Fish:	nz50Tg + MO3-csf3r gz32Tg; nz50Tg gz32Tg; nz50Tg + MO1-irf8
Condition:	chemical treatment: doxycycline monohydrate
Knockdown Reagents:	MO1-irf8 MO3-csf3r
Observed In:	liver liver neutrophil
Stage:	Day 6

Fig. 3

Effect of morpholino suppression of neutrophil differentiation and morpholino-mediated expansion of neutrophil population on liver size. (A–C) Representative images of 6 dpf kras-/lyz+ and kras+/lyz+ larvae injected with different morpholinos: Mo-SC (A), Mo-gcsfr (B) or Mo-irf8 (C). (D–F) Neutrophil counts (D) and density (E) in the liver area and liver size (F) after morpholino injection. n >15 in each group. Statistical significant: ^*p <0.05.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
DsRed2	gz32Tg; nz50Tg	chemical treatment: doxycycline monohydrate	Day 6	neutrophil	IFL
	gz32Tg; nz50Tg + MO1-irf8	chemical treatment: doxycycline monohydrate	Day 6	neutrophil	IFL
	gz32Tg; nz50Tg + MO3-csf3r	chemical treatment: doxycycline monohydrate	Day 6	neutrophil	IFL
	nz50Tg	chemical treatment: doxycycline monohydrate	Day 6	neutrophil	IFL
	nz50Tg + MO1-irf8	chemical treatment: doxycycline monohydrate	Day 6	neutrophil	IFL
	nz50Tg + MO3-csf3r	chemical treatment: doxycycline monohydrate	Day 6	neutrophil	IFL
EGFP	gz32Tg; nz50Tg	chemical treatment: doxycycline monohydrate	Day 6	liver	IFL
	gz32Tg; nz50Tg + MO1-irf8	chemical treatment: doxycycline monohydrate	Day 6	liver	IFL
	gz32Tg; nz50Tg + MO3-csf3r	chemical treatment: doxycycline monohydrate	Day 6	liver	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
gz32Tg; nz50Tg	chemical treatment: doxycycline monohydrate	Day 6	liver increased size, abnormal
		Day 6	liver neutrophil increased amount, abnormal
gz32Tg; nz50Tg + MO1-irf8	chemical treatment: doxycycline monohydrate	Day 6	liver increased size, abnormal
		Day 6	liver neutrophil increased amount, abnormal
nz50Tg + MO3-csf3r	chemical treatment: doxycycline monohydrate	Day 6	liver neutrophil decreased amount, abnormal

Acknowledgments:
ZFIN wishes to thank the journal Journal of hepatology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from Journal of hepatology, 63(2), Yan, C., Huo, X., Wang, S., Feng, Y., Gong, Z., Stimulation of hepatocarcinogenesis by neutrophils upon induction of oncogenic kras expression in transgenic zebrafish, 420-8, Copyright (2015) with permission from Elsevier. Full text @ J. Hepatol.

Acknowledgments:

ZFIN wishes to thank the journal Journal of hepatology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Journal of hepatology, 63(2), Yan, C., Huo, X., Wang, S., Feng, Y., Gong, Z., Stimulation of hepatocarcinogenesis by neutrophils upon induction of oncogenic kras expression in transgenic zebrafish, 420-8, Copyright (2015) with permission from Elsevier. Full text @ J. Hepatol.