FIGURE

Fig. 4

ID

ZDB-FIG-150803-9

Publication

Bhatia et al., 2015 - Functional Assessment of Disease-Associated Regulatory Variants In Vivo Using a Versatile Dual Colour Transgenesis Strategy in Zebrafish

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Fig. 4

Dual-fluorescence transgenic analysis of IRF6 enhancer MCS-9.7 where a SNP (rs642961, G>A) in a TFAP2A (AP2α) binding site has been associated with cleft lip.

(A) Regulatory landscape of IRF6, depicting the location of MCS-9.7 enhancer. The conservation plot on the right shows the absence of sequence conservation for MCS-9.7 in the zebrafish genome. (B) IRF6-MCS-9.7 enhancer-driven F1 reporter transgenics. The Wt(G) allele drives expression in the first pharyngeal arch (PA1) (arrow) and in the developing ethmoid plate (EP) (curved arrow). Mut(A) has lost EP expression but maintains PA1 expression. (C) RNA in situ hybridisation analysis of zebrafish irf6 at 72hpf showing overlap of the reporter gene expression domain driven by Wt(G) allele with the endogenous irf6 expression pattern in the developing jaw. Wt: wild-type; Mut: mutant; hpf: hours post fertilization.

Expression Data

Genes:	EGFP irf6 mCherry
Fish:	WIK/AB zf1006Tg; zf1007Tg (WIK/AB)
Anatomical Terms:	ethmoid cartilage pharyngeal arch 1
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf1006Tg; zf1007Tg (WIK/AB)
Observed In:	ethmoid cartilage
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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