|ZFIN ID: ZDB-FIG-150629-7|
|Stage Range:||Prim-5 to Day 4|
|Stage Range:||Prim-5 to Day 4|
Characterization of the NO067 Zebrafish Mutant
(A and B) WISH for apo-E in a sibling (A) and NO067 mutant (B) brain at 4 days post fertilization is shown. White arrows indicate apo-E+ microglia.
(C and D) WISH for mpeg1 in a sibling (C) and NO067 mutant (D) embryo at 4 days post fertilization is shown.
(E, H, and J) Lateral view shows a representative pU1::TagRFP wild-type (E), mutant (H), and slc7a7 morphant (J) at 24 hpf.
(F, I, and K) Lateral view shows a representative mpeg1::Kaede wild-type (F), mpeg1::Kaede morphant (K), and mpeg1::eGFP mutant (I) at 24 hpf.
(G and L) Numbers of leukocytes and macrophages in siblings and mutants (G) and wild-type and slc7a7 morphants (L) at 24 hpf are shown.
(M) Representative time-lapse images show two leukocytes undergoing apoptosis within the head region of a NO067;pU.1::TagRFP embryo (white arrowheads). Dotted line indicates the shape of the head and the eye.
(N–Q) Numbers of leukocytes (N and O) and macrophages (P and Q) in siblings and mutants in the head (N and P) and rest of the body (O and Q) at 2 days post fertilization are shown, as indicated in yellow in the schematics. p < 0.0005, Mann-Whitney test.
Scale bars for all images, 25 µm. Sib, sibling; n, number of embryos. See also Figures S2 and S3 and Movie S1.
|apoeb||WT||standard conditions||Day 4||ISH|
|slc7a7t30713/t30713||standard conditions||Day 4||Not Detected||brain||ISH|
|Kaede||gl24Tg; s1999tTg||standard conditions||Prim-5||macrophage||IFL|
|gl24Tg; s1999tTg + MO1-slc7a7||standard conditions||Prim-5||macrophage||IFL|
|slc7a7t30713/t30713; gl24Tg; s1999tTg||standard conditions||Prim-5||macrophage||IFL|
|mpeg1.1||WT||standard conditions||Day 4||macrophage||ISH|
|slc7a7t30713/t30713||standard conditions||Day 4||macrophage||ISH|
|hdb2Tg + MO1-slc7a7||standard conditions||Prim-5||leukocyte||IFL|
|slc7a7t30713/t30713; hdb2Tg||standard conditions||Prim-5||leukocyte||IFL|
|gl24Tg; s1999tTg + MO1-slc7a7||standard conditions||Prim-5||whole organism has normal numbers of parts of type macrophage, normal|
|hdb2Tg + MO1-slc7a7||standard conditions||Prim-5||whole organism has normal numbers of parts of type leukocyte, normal|
|slc7a7t30713/t30713||standard conditions||Prim-5||whole organism has normal numbers of parts of type leukocyte, normal|
|Prim-5||whole organism has normal numbers of parts of type macrophage, normal|
|Long-pec||head has fewer parts of type leukocyte, abnormal|
|Long-pec||head has fewer parts of type macrophage, abnormal|
|Long-pec||trunk has normal numbers of parts of type leukocyte, normal|
|Long-pec||trunk has normal numbers of parts of type macrophage, normal|
|Day 4||brain lacks all parts of type microglial cell, abnormal|
|Day 4||microglia development decreased occurrence, abnormal|
|slc7a7t30713/t30713; hdb2Tg||standard conditions||Long-pec||head leukocyte apoptotic, abnormal|
|Acknowledgments:||ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.|