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ZIRC
ZFIN ID: ZDB-FIG-150629-7
Rossi et al., 2015 - The SLC7A7 Transporter Identifies Microglial Precursors prior to Entry into the Brain. Cell Reports   11(7):1008-17 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Day 4

Fig. 2

Characterization of the NO067 Zebrafish Mutant

(A and B) WISH for apo-E in a sibling (A) and NO067 mutant (B) brain at 4 days post fertilization is shown. White arrows indicate apo-E+ microglia.

(C and D) WISH for mpeg1 in a sibling (C) and NO067 mutant (D) embryo at 4 days post fertilization is shown.

(E, H, and J) Lateral view shows a representative pU1::TagRFP wild-type (E), mutant (H), and slc7a7 morphant (J) at 24 hpf.

(F, I, and K) Lateral view shows a representative mpeg1::Kaede wild-type (F), mpeg1::Kaede morphant (K), and mpeg1::eGFP mutant (I) at 24 hpf.

(G and L) Numbers of leukocytes and macrophages in siblings and mutants (G) and wild-type and slc7a7 morphants (L) at 24 hpf are shown.

(M) Representative time-lapse images show two leukocytes undergoing apoptosis within the head region of a NO067;pU.1::TagRFP embryo (white arrowheads). Dotted line indicates the shape of the head and the eye.

(N–Q) Numbers of leukocytes (N and O) and macrophages (P and Q) in siblings and mutants in the head (N and P) and rest of the body (O and Q) at 2 days post fertilization are shown, as indicated in yellow in the schematics. p < 0.0005, Mann-Whitney test.

Scale bars for all images, 25 µm. Sib, sibling; n, number of embryos. See also Figures S2 and S3 and Movie S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
apoeb WT standard conditions Day 4 brain microglial cell ISH
slc7a7t30713/t30713 standard conditions Day 4 Not Detected brain ISH
Kaede gl24Tg; s1999tTg standard conditions Prim-5 macrophage IFL
gl24Tg; s1999tTg + MO1-slc7a7 standard conditions Prim-5 macrophage IFL
slc7a7t30713/t30713; gl24Tg; s1999tTg standard conditions Prim-5 macrophage IFL
mpeg1.1 WT standard conditions Day 4 macrophage ISH
slc7a7t30713/t30713 standard conditions Day 4 macrophage ISH
TagRFP hdb2Tg standard conditions Prim-5 leukocyte IFL
hdb2Tg + MO1-slc7a7 standard conditions Prim-5 leukocyte IFL
slc7a7t30713/t30713; hdb2Tg standard conditions Prim-5 leukocyte IFL
Long-pec head leukocyte IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gl24Tg; s1999tTg + MO1-slc7a7 standard conditions Prim-5 whole organism has normal numbers of parts of type macrophage, normal
hdb2Tg + MO1-slc7a7 standard conditions Prim-5 whole organism has normal numbers of parts of type leukocyte, normal
slc7a7t30713/t30713 standard conditions Prim-5 whole organism has normal numbers of parts of type leukocyte, normal
Prim-5 whole organism has normal numbers of parts of type macrophage, normal
Long-pec head has fewer parts of type leukocyte, abnormal
Long-pec head has fewer parts of type macrophage, abnormal
Long-pec trunk has normal numbers of parts of type leukocyte, normal
Long-pec trunk has normal numbers of parts of type macrophage, normal
Day 4 brain lacks all parts of type microglial cell, abnormal
Day 4 microglia development decreased occurrence, abnormal
slc7a7t30713/t30713; hdb2Tg standard conditions Long-pec head leukocyte apoptotic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.