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ZIRC
ZFIN ID: ZDB-FIG-150617-1
Wu et al., 2015 - Hypoxia-Induced Retinal Neovascularization in Zebrafish Embryos: A Potential Model of Retinopathy of Prematurity. PLoS One   10:e0126750 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Protruding-mouth

Fig. 1

CoCl2 induces abnormal neovascularization in zebrafish embryos.

(A) Morphological images obtained by an optical microscope revealed no severe phenotype in CoCl2-treated Tg(fli1a:EGFP) embryos at 3 dpf. (B) With fluorescence excitation, ectopic SIV and excessive retinal vascularization are shown in CoCl2-treated embryos compared with the untreated control. (C, D) A dose-dependent decrease in survival rates (four independent experiments; n = 35 in each group) in embryos treated with increasing concentrations (0–20 mM) and an increase in the vascular defect occurrence rate in the SIV and retinal vessels are shown (three independent experiments; n = 35 in each group). (E, F) Real-time RT-PCR data show that CoCl2 treatment causes overexpression of vegfaa and vegfr2 mRNAs in zebrafish. (G,H) Absolute quantification of the copy number of vegfr2 and plxnd1 by real-time PCR are reduced at 5 dpf versus 3 dpf. Each bar represents the mean ± SEM. * (p < 0.01) and ** (p < 0.001) compared with the mock control group. SIV, subintestinal vessel; RV, retinal vessel.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Protruding-mouth retina ocular blood vessel IFL
Protruding-mouth subintestinal vein IFL
Protruding-mouth trunk vasculature IFL
y1Tg chemical treatment: cobalt dichloride Protruding-mouth retina ocular blood vessel IFL
Protruding-mouth subintestinal vein IFL
Protruding-mouth trunk vasculature IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg chemical treatment: cobalt dichloride Protruding-mouth retina ocular blood vessel increased amount, abnormal
Protruding-mouth retina ocular blood vessel increased branchiness, abnormal
Protruding-mouth retina vasculature development in camera-type eye increased process quality, abnormal
Protruding-mouth retina vasculature development in camera-type eye process quality, abnormal
Protruding-mouth subintestinal vein malformed, abnormal
Protruding-mouth subintestinal vein blood vessel mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One