ZFIN ID: ZDB-FIG-150615-3
Krock et al., 2014 - The Par-PrkC Polarity Complex Is Required for Cilia Growth in Zebrafish Photoreceptors. PLoS One   9:e104661 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 5

Fig. 3 Effects of pard3 suppression on apical determinants. Immunohistochemistry was performed on transverse cryosections of wild-type and pard3 morphants. Phalloidin was used to label actin (red) in all panels. (A–B) Transverse cryosections of wild-type and pard3 morphants stained with phalloidin (red) and PrkCi immunoreactivity (green). Arrows mark longitudinal fibers in the ellipsoid. (C–D) Cryosections stained with phalloidin (red) and Crb immunoreactivity (green). (E–F) Cryosections stained with phalloidin (red) and ZO-1 immunoreactivity (green). Sections were also counterstained with DAPI (blue). ONL = outer nuclear layer; OPL = outer plexiform layer; INL = inner nuclear layer. Scale bar = 10 µm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-crb IHC AB/EKW standard conditions Day 5 eye photoreceptor cell apical region
IHC Day 5 outer limiting membrane
IHC AB/EKW + MO1-pard3ab standard conditions Day 5 outer limiting membrane
Ab1-prkcz IHC AB/EKW standard conditions Day 5 eye photoreceptor cell apical region
IHC Day 5 outer limiting membrane
IHC AB/EKW + MO1-pard3ab standard conditions Day 5 outer limiting membrane
Ab1-tjp1 IHC AB/EKW standard conditions Day 5 outer limiting membrane
IHC AB/EKW + MO1-pard3ab standard conditions Day 5 outer limiting membrane
Phenotype Details
Fish Conditions Stage Phenotype
AB/EKW + MO1-pard3ab standard conditions Day 5 eye photoreceptor cell apical region decreased area, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One