FIGURE

Fig. 4

ID
ZDB-FIG-150423-31
Publication
Yousfi et al., 2015 - The Impact of Tumor Nitric Oxide Production on VEGFA Expression and Tumor Growth in a Zebrafish Rat Glioma Xenograft Model
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Fig. 4

Alkaline phosphatase assay of xenografted DAF-FM-DA treated embryos.

Embryos were injected with red CM-Dil labeled tumor cells, incubated at 4 dpi in DAF (5µM) and fixed at 4dpi before detection of alkaline phosphatase activity. (A) Bright field image of the embryos before treatments. (B) Neovascularisation was observed in 12 of 14 (85.5%) embryos presenting NO production in tumor environment. (D) Fluorescence detection of glioma cells, (C) DAF signal, (E) merge. The white arrows indicate the part of the tumor mass that co-localizes with DAF fluorescence signal. (F) A control embryo with absence of vessels in this region of the yolk.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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