ZFIN Figure: Navis et al., 2015, Fig. 1

ZFIN ID: ZDB-FIG-150421-4

ZFIN ID: ZDB-FIG-150421-4

Navis et al., 2015 - Loss of cftr function leads to pancreatic destruction in larval zebrafish. Developmental Biology 399(2):237-48 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	cftr DsRed EGFP GFP RFP
Antibody:	zn-5
Fish:	AB pd1041Tg gz15Tg; pd1042Tg m1018Tg; pd1041Tg
Anatomical Terms:	liver pancreas pancreatic acinar cell pancreatic duct primary islet
Stage Range:	Protruding-mouth to Day 6

Fig. 1

cftr is expressed in the pancreatic duct. (A–D) Wholemount in situ hybridization for cftr. (A) Dorsal and (B) lateral view of cftr expression at 3 dpf. (C) Dorsal and (D) lateral view of cftr expression at 5 dpf. (E and F) Transverse section of TgBAC(cftr-GFP) expressed in the pancreatic duct at (E) 3 dpf in conjunction with ptf1a expression in the acinar cells of the pancreas and (F) at 5 dpf in conjunction with ZN-5, an antibody that marks the pancreas. (G) Live, confocal image of TgBAC(cftr-GFP) expression along the length of the pancreatic duct in conjunction with Tg(ins:dsRed) to mark the β cells of the principal islet at 5 dpf. (H) Live confocal image of ductal TgBAC(cftr-RFP) expression in conjunction with ela:GFP to indicate the acinar tissue. White arrows indicate cftr expression in the pancreatic duct. ib: intestinal bulb, ov: otic vesicle, p: pancreas. (A–D) Scale bars=100 µm, (E–H) Scale bars=50 µm.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
cftr	AB	standard conditions	Protruding-mouth	pancreas	ISH
			Day 5	pancreatic duct	ISH
DsRed	gz15Tg; pd1042Tg	standard conditions	Day 6	liver	IFL
	m1018Tg; pd1041Tg	standard conditions	Day 6	primary islet	IFL
EGFP	gz15Tg; pd1042Tg	standard conditions	Day 6	pancreatic acinar cell	IFL
GFP	m1018Tg; pd1041Tg	standard conditions	Day 6	pancreatic duct	IFL
	pd1041Tg	standard conditions	Day 5	pancreatic duct	IFL
RFP	gz15Tg; pd1042Tg	standard conditions	Day 6	pancreatic duct	IFL

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Anatomy
zn-5	IHC	pd1041Tg	standard conditions	Day 5	pancreas

Phenotype Details No data available

Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from Developmental Biology, 399(2), Navis, A., Bagnat, M., Loss of cftr function leads to pancreatic destruction in larval zebrafish, 237-48, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.