ZFIN ID: ZDB-FIG-150421-4
Navis et al., 2015 - Loss of cftr function leads to pancreatic destruction in larval zebrafish. Developmental Biology   399(2):237-48 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 6

Fig. 1

cftr is expressed in the pancreatic duct. (A–D) Wholemount in situ hybridization for cftr. (A) Dorsal and (B) lateral view of cftr expression at 3 dpf. (C) Dorsal and (D) lateral view of cftr expression at 5 dpf. (E and F) Transverse section of TgBAC(cftr-GFP) expressed in the pancreatic duct at (E) 3 dpf in conjunction with ptf1a expression in the acinar cells of the pancreas and (F) at 5 dpf in conjunction with ZN-5, an antibody that marks the pancreas. (G) Live, confocal image of TgBAC(cftr-GFP) expression along the length of the pancreatic duct in conjunction with Tg(ins:dsRed) to mark the β cells of the principal islet at 5 dpf. (H) Live confocal image of ductal TgBAC(cftr-RFP) expression in conjunction with ela:GFP to indicate the acinar tissue. White arrows indicate cftr expression in the pancreatic duct. ib: intestinal bulb, ov: otic vesicle, p: pancreas. (A–D) Scale bars=100 µm, (E–H) Scale bars=50 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cftr AB standard conditions Protruding-mouth pancreas ISH
Day 5 pancreatic duct ISH
DsRed gz15Tg; pd1042Tg standard conditions Day 6 liver IFL
m1018Tg; pd1041Tg standard conditions Day 6 primary islet IFL
EGFP gz15Tg; pd1042Tg standard conditions Day 6 pancreatic acinar cell IFL
GFP m1018Tg; pd1041Tg standard conditions Day 6 pancreatic duct IFL
pd1041Tg standard conditions Day 5 pancreatic duct IFL
RFP gz15Tg; pd1042Tg standard conditions Day 6 pancreatic duct IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-5 IHC pd1041Tg standard conditions Day 5 pancreas
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 399(2), Navis, A., Bagnat, M., Loss of cftr function leads to pancreatic destruction in larval zebrafish, 237-48, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.