FIGURE

Fig. 7

ID

ZDB-FIG-150408-32

Publication

Merkes et al., 2015 - Ewing Sarcoma Ewsa Protein Regulates Chondrogenesis of Meckel's Cartilage through Modulation of Sox9 in Zebrafish

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Fig. 7

Ewsa binds to ctgfa and ctgfb target genes.

A. Lateral views (anterior to the left, dorsal to the top) of 4dpf of (Left) wt/wt and (Right) MZ ewsa/ewsa visualized by in situ hybridization using probe for ctgfa (tom panel) and ctgfb (bottom panel). m: Meckel′s cartilage. B. Ventral views (anterior to the left) of 4dpf Meckel′s cartilage of (Left) wt/wt and (Right) MZ ewsa/ewsa visualized by immunohistochemistry using anti-Ctgf antibody (green), and anti-Collagen type II antibody (Red). C. (left) Schematics of ctgfa and ctgfb genes. Black circle: Sox9 binding site, black square: exon, arrows: PCR primer for ChIP assay. (right) ChIP assays were performed using 27 hpf zebrafish embryos. IgG: negative control of ChIP, anti-Ewsa: ChIP sample using EWSa antibody, input: 4.5% of DNA from total lysates was subjected to PCR, dw: negative control for PCR reaction.

Expression Data

Genes:	ccn2a ccn2b
Antibody:	Ab1-ctgf
Fish:	AB ewsr1a^{zm00260171Tg/zm00260171Tg}
Anatomical Terms:	Meckel's cartilage Meckel's cartilage chondrocyte
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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