header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-150316-24
Irion et al., 2014 - Precise and efficient genome editing in zebrafish using the CRISPR/Cas9 system. Development (Cambridge, England)   141(24):4827-30 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 2

Homology-directed repair at the alb locus. (A) Donor DNAs used. The PCR fragments were also cloned into pGEM-T and injected as circular DNA molecules; the construct with the two CRISPR target sites is depicted. (B-D) Dorsal views of larvae 5dpf: uninjected albb4 control (B), low efficiency repair using linear donor DNA (C) and high efficiency repair using circular donor DNA (D). (E,F) Two examples of adult F0 fish showing pigmented melanophores as a consequence of HDR in melanophore stem cells.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
slc45a2b4/b4 standard conditions Day 5 melanocyte decreased pigmentation, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development