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General Information
ZIRC
ZFIN ID: ZDB-FIG-150316-23
Irion et al., 2014 - Precise and efficient genome editing in zebrafish using the CRISPR/Cas9 system. Development (Cambridge, England)   141(24):4827-30 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 5

Fig. 1

CRISPR-mediated alb knockout. (A) Schematic representation of the alb locus. The gene consists of seven exons (introns are not drawn to scale). The mutation albb4 introduces a premature stop codon into exon 6. (B) The sequence of exon 6 (in capitals). The CRISPR target site, PAM motif and the albb4 mutation are indicated; the SNPs introduced into the donor DNA fragments are shown beneath the CRISPR target site. (C-F) Dorsal views of larvae at 5dpf: uninjected control wild type (C) and albb4 (D) larvae, and injected wild-type larvae with moderate (E) and good (F) alb knockout efficiency.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + CRISPR1-slc45a2 standard conditions Day 5 melanocyte decreased pigmentation, abnormal
slc45a2b4/b4 standard conditions Day 5 melanocyte decreased pigmentation, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development