FIGURE

Fig. 1

ID

ZDB-FIG-150202-11

Publication

Verleyen et al., 2014 - Orphan G-Protein Coupled Receptor 22 (Gpr22) Regulates Cilia Length and Structure in the Zebrafish Kupffer's Vesicle

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Fig. 1

The downwards bending of the tail in gpr22 morphants is rescued by the co-injection of gpr22 mRNA.

(A–C) Lateral views with anterior to the left of zebrafish embryos at 24 hpf injected at one cell stage with (A) water, (B) 0.2 mM fluorescein tagged gpr22 MO, (C) 75 pg/nl gpr22 mRNA or (D) 0.2 mM fluorescein tagged gpr22 MO and 100 pg/nl gpr22 mRNA. (B, C) Deregulation of gpr22 results in a bending of the tail, a reduction in length of the anterior-posterior axis and heart edema (arrow). (E) Quantification of the tail phenotype. (D, E) The curly tail of embryos injected with fluorescein tagged gpr22 MO is rescued by the co-injection of gpr22 mRNA in a dose-dependent manner. MO = morpholino, hpf = hours post-fertilization, n = number of analyzed embryos, WT = wild type.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-gpr22a + MO4-tp53
Knockdown Reagents:	MO1-gpr22a MO4-tp53
Observed In:	caudal fin heart whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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