ZFIN ID: ZDB-FIG-141125-4
Choksi et al., 2014 - Systematic discovery of novel ciliary genes through functional genomics in the zebrafish. Development (Cambridge, England)   141:3410-9 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 20-25 somites to Day 4

Fig. 3

Morpholino knockdown of FIGs causes multiple ciliary dysfunction-associated phenotypes in zebrafish embryos. Five ciliary phenotypes were scored in the zebrafish embryos injected with morpholinos targeting the 50 selected FIGs. The extent of body axis curvature: (A) wild type or (B) curved body. Otolith defects in the inner ear: (C) two otoliths, (D) greater than two otholiths or (E) a single otolith. Swelling of the brain ventricles (hydrocephalus): (F) normal or (G) hydrocephalus. Kidney cysts: (H) no cysts or (I) a minimum of one cyst per embryo. Expression of lefty2: (J) leftward expression, (K) rightward expression or (L) bilateral expression or no expression (data not shown). The red arrowhead indicates the position of a kidney cyst (I); the black arrowheads indicate the midline (J-L). Scale bars: 100μm in A,F,H; 20μm in C,J. (M) Morpholino knockdowns of 31 genes exhibited defects in at least two of the assayed phenotypes. For morphological phenotypes, each square represents at least 60 assayed embryos from two independent injections. For left-right asymmetry defects, each square represents a minimum of 30 stained and scored embryos. A red square indicates that the morphant phenotype level was significantly different from the wild type and met a minimum defective percentage (see Materials and Methods).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-afg1lb standard conditions Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-aftphb standard conditions Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-ak9 standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-arhgef18b standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-ces3 standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-cfap20dc standard conditions 20-25 somites otolith morphology, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-clic6 standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-cp standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-crocc2 standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
Day 4 pronephros cystic, abnormal
AB + MO1-fgfbp2b standard conditions Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-gb:co360592 standard conditions Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-hhipl1 standard conditions Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-kcnip1a standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-lect2.1 standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-magi3b standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-mustn1a standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-plcxd2 standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-scg5 standard conditions Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-si:ch211-195b15.7 standard conditions Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-si:ch211-212d10.1 standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-si:ch211-71m22.1 standard conditions Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
Day 4 pronephros cystic, abnormal
AB + MO1-si:dkey-165a24.9 standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
Day 4 pronephros cystic, abnormal
AB + MO1-si:dkey-26i13.8 standard conditions 26+ somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
Day 4 pronephros cystic, abnormal
AB + MO1-slc43a3a standard conditions Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
Day 4 pronephros cystic, abnormal
AB + MO1-spa17 standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-tmc5 standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
Day 4 pronephros cystic, abnormal
AB + MO1-tp53bp2b standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO1-zbbx standard conditions Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
AB + MO2-mns1 standard conditions 20-25 somites otolith morphology, abnormal
Prim-5 determination of left/right symmetry disrupted, abnormal
Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
Day 4 pronephros cystic, abnormal
AB + MO3-cfap161 standard conditions Long-pec ventricular system hydrocephalic, abnormal
Long-pec whole organism anterior-posterior axis curved, abnormal
Acknowledgments:
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