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ZFIN ID: ZDB-FIG-141016-46
Hudish et al., 2013 - miR-219 Regulates Neural Precursor Differentiation by Direct Inhibition of Apical Par Polarity Proteins. Developmental Cell   27(4):387-398 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 7

Blocking miR-219 Access to pard3 or prkci 32 UTRs Phenocopies miR-219 Loss of Function

All panels show representative images of spinal cord transverse sections with dorsal up.

(A–C) Larvae (3 dpf) labeled to detect Prcki localization. In the control, Prkci is limited to a small central canal (A). By contrast, Prkci extends dorsally in pard3 TP MO and prkci TP MO-injected larvae, marking a primitive lument (B and C).

(D–F) Similarly to Prcki, ZO-1 is restricted to a central canal in the control larva (D) but extends more dorsally in pard3 TP MO and prkci TP MO-injected larvae (E and F).

(G–I) pard3 TP MO and prkci TP MO-injected larvae have fewer medial spinal cord radial glia than control.

(J–L) pard3 TP MO and prkci TP MO-injected larvae have more Sox2+ cells in dorsal spinal cord than control.

(M–O) BrdU incorporation at 3 dpf. TP MO-injected larvae have more labeled cells than control.

(P) Graph showing number of BrdU+ cells in dorsal, ventral, and entire spinal cord. Data represent ± SEM (n = 10 larvae for each experiment, five to ten section per larva). p < 0.05, p < 0.005, p < 0.0005, unpaired t test. Scale bar equals 10 µm.

See also Figure S2.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-prkcz IHC WT control Protruding-mouth lateral wall spinal cord apical plasma membrane
IHC WT + MO3-prkci standard conditions Protruding-mouth lateral wall spinal cord apical plasma membrane
IHC WT + MO8-pard3ab standard conditions Protruding-mouth lateral wall spinal cord apical plasma membrane
Ab1-tjp1 IHC WT control Protruding-mouth lateral wall spinal cord apical plasma membrane
IHC WT + MO3-prkci standard conditions Protruding-mouth lateral wall spinal cord apical plasma membrane
IHC WT + MO8-pard3ab standard conditions Protruding-mouth lateral wall spinal cord apical plasma membrane
Ab3-sox2 IHC WT control Protruding-mouth lateral wall spinal cord cell
IHC WT + MO3-prkci standard conditions Protruding-mouth lateral wall spinal cord cell
IHC WT + MO8-pard3ab standard conditions Protruding-mouth lateral wall spinal cord cell
zrf-1 IHC WT control Protruding-mouth spinal cord radial glial cell
IHC WT + MO3-prkci standard conditions Protruding-mouth spinal cord radial glial cell
IHC WT + MO8-pard3ab standard conditions Protruding-mouth spinal cord radial glial cell
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-prkci standard conditions Protruding-mouth central canal increased size, abnormal
Protruding-mouth spinal cord has fewer parts of type radial glial cell, abnormal
WT + MO8-pard3ab standard conditions Protruding-mouth central canal increased size, abnormal
Protruding-mouth spinal cord has fewer parts of type radial glial cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 27(4), Hudish, L.I., Blasky, A.J., and Appel, B., miR-219 Regulates Neural Precursor Differentiation by Direct Inhibition of Apical Par Polarity Proteins, 387-398, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell