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ZIRC
ZFIN ID: ZDB-FIG-140910-25
Heijnen et al., 2014 - Ribosomal Protein Mutations Induce Autophagy through S6 Kinase Inhibition of the Insulin Pathway. PLoS Genetics   10:e1004371 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 4 RP loss increases phospo-S6 kinase signaling. (A) Western blot analysis showing the expression of phosphorylated S6 kinase in GFP-LC3 HEK cells transfected with siScr or siRPS19 and either untreated or treated with 100 nM rapamycin overnight. (B) Western blot analysis of phosphorylated S6 kinase expression in CD34+ cord blood cells infected with a control shRNA (shScr) or an shRNA against RPS19 (shRPS19). (C) Western blot analysis of phosphorylated S6 kinase expression in LCLs derived from a normal control or DBA patients. (D) Western blot analysis of phosphorylated S6 kinase expression in 2 dpf zebrafish embryos.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab6-rps6k WB WT control Long-pec whole organism
WB rpl11hi3820bTg/hi3820bTg control Long-pec whole organism
WB rps3ahi1290Tg/hi1290Tg control Long-pec whole organism
WB rps7hi1034bTg/hi1034bTg control Long-pec whole organism
Phenotype Details
Fish Conditions Stage Phenotype
rpl11hi3820bTg/hi3820bTg control Long-pec ribosomal protein S6 kinase activity increased occurrence, abnormal
rps3ahi1290Tg/hi1290Tg control Long-pec ribosomal protein S6 kinase activity increased occurrence, abnormal
rps7hi1034bTg/hi1034bTg control Long-pec ribosomal protein S6 kinase activity increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.