ZFIN ID: ZDB-FIG-140811-22
Ju et al., 2014 - Activation of Sonic hedgehog signaling in neural progenitor cells promotes glioma development in the zebrafish optic pathway. Oncogenesis   3:e96 Full text @ Oncogenesis
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 5

Differentially expressed genes and a radial glia cell signature of the zOPGs. (a) A heatmap generated by hierarchical clustering showing differentially expressed genes for wild-type (WT) and retinal tumors, respectively. The color scale (Z-score) is denoted on the right, with red showing upregulation and green showing downregulation. A subset of upregulated genes is listed on the right. (b) Upregulation of gli1, gli2a, gfap and wnt5a was confirmed by real-time quantitative PCR (qPCR). Microarray data represent the average expression from four eye tumors versus four control eyes. qPCR data are from the same four samples performed in quadruplicate. All data were normalized to actin b1expression. Error bars show s.d. (cg2) Series of cryosections from a single fish with typical zOPG showed overexpression of the astrocyte/radial glia markers of Pax2 (c, c2), GFAP (d, d2), S100β (e, e2), BLBP (f, f2) and the stem cell and glioma marker Sox2 (g, g2) as compared with their respective expression in the ONH of an age-matched WT fish. Fragmented lines demarcate the ONH region. Scale bars, 40μm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pax2 IHC WT standard conditions Adult astrocyte
IHC Adult radial glial cell
IHC sj4Tg standard conditions Adult astrocyte
IHC Adult optic nerve head glioma
IHC Adult radial glial cell
Ab1-s100 IHC WT standard conditions Adult astrocyte
IHC Adult radial glial cell
IHC sj4Tg standard conditions Adult astrocyte
IHC Adult optic nerve head glioma
IHC Adult radial glial cell
Ab1-sox2 IHC WT standard conditions Adult somatic stem cell
IHC sj4Tg standard conditions Adult optic nerve head glioma
IHC Adult somatic stem cell
Ab2-fabp7 IHC WT standard conditions Adult astrocyte
IHC Adult radial glial cell
IHC sj4Tg standard conditions Adult astrocyte
IHC Adult optic nerve head glioma
IHC Adult radial glial cell
Ab2-gfap IHC WT standard conditions Adult astrocyte
IHC Adult radial glial cell
IHC sj4Tg standard conditions Adult astrocyte
IHC Adult optic nerve head glioma
IHC Adult radial glial cell
Phenotype Details
Fish Conditions Stage Phenotype
sj4Tg standard conditions Adult eye glioma increased object quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Oncogenesis for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Oncogenesis