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ZFIN ID: ZDB-FIG-140807-34
Araya et al., 2014 - Mesoderm is required for coordinated cell movements within zebrafish neural plate in vivo. Neural Development   9(1):9 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 3 Lack of mesoderm disrupts neural tube morphogenesis in both anterior and posterior levels of the neuro-axis. (A,B) Projection of confocal z-series showing dorsal view of mesoderm (red), expressing the muscle marker (MF-20) and aPKC (green) in caudal hindbrain and anterior spinal cord at 24 hpf. The normal midline domain of aPKC is maintained in regions of MZoep embryo that are adjacent to mesoderm (arrowhead). (C) Mesoderm is still present but disrupted in spt-deficient embryos and the aPKC domain is largely normal. (D) Embryos deficient for both oep and spt have no mesoderm adjacent to the spinal cord and apical aPKC is severely disrupted in spinal cord (arrowheads). Basal extremity of neural tube indicated by yellow dotted lines. aPKC, atypical protein kinase C; hpf, hours post fertilization; MZoep, maternal-zygotic one-eyed pinhead.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-MF20 IHC WT standard conditions Prim-5 mesoderm
IHC WT + MO1-tbx16 + MO2-tbx16 standard conditions Prim-5 mesoderm
IHC tdgf1tz257/tz257 standard conditions Prim-5 mesoderm
IHC tdgf1tz257/tz257 + MO1-tbx16 + MO2-tbx16 standard conditions Prim-5 Not Detected mesoderm
Ab1-prkca IHC WT standard conditions Prim-5 hindbrain posterior side
IHC Prim-5 spinal cord anterior region
IHC WT + MO1-tbx16 + MO2-tbx16 standard conditions Prim-5 hindbrain posterior side
IHC Prim-5 spinal cord anterior region
IHC tdgf1tz257/tz257 standard conditions Prim-5 hindbrain posterior side
IHC Prim-5 spinal cord anterior region
IHC tdgf1tz257/tz257 + MO1-tbx16 + MO2-tbx16 standard conditions Prim-5 hindbrain posterior side
IHC Prim-5 spinal cord anterior region
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tbx16 + MO2-tbx16 standard conditions Prim-5 mesoderm decreased amount, abnormal
tdgf1tz257/tz257 + MO1-tbx16 + MO2-tbx16 standard conditions Prim-5 mesoderm absent, abnormal
Prim-5 neural tube morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.