Fig. 5

Scrib and Vangl2, but not Nhsl1b or Pk1b, are required for neuroepithelial cell polarity. (A-E) Confocal images showing floorplate planar polarity in 33 hours post-fertilization (hpf) zebrafish embryos. Anterior is to the top. ZO-1 marks subapical tight junctions (red), γ-tubulin marks basal bodies (red, indicated by arrows in A,B) and Arl13b marks the axonemes of primary cilia (green). Whereas basal bodies are localized to the posterior side of floorplate cells in wild type (A), nhsl1b^fh131 mutants (D) and pk1b morphants (E) they are centrally located in zygotic vangl2^m209 mutants (B) which have a widened floorplate due to defective neural tube convergence and in zygotic scrib^rw468 mutants (C), which have only a mild neural tube convergence defect. (F) Quantification of the percentage of cells displaying an anterior, central or posterior position of basal bodies in floorplate cells. Asterisk indicates statistically significant difference from wild type (WT) as determined by χ² test, P<0.0001. (G,H) Live confocal imaging (dorsal view, anterior to the top) of mosaically expressed GFP-Pk marking anterior membranes (arrows) of neuroepithelial progenitors in 16 hpf wild-type (A) and nhsl1b^fh131 mutant (B) embryos. Scale bars: 10 µm.