Fig. 5
A Calcineurin-Mediated Mechanism Regulates Growth (A) Adult caudal fin before amputation and DMSO treatment. (B) Amputated fin at 0 days dpa. (C) DMSO-treated fin at 38 dpa. (D) Adult fin before amputation and FK506 treatment. (E) Amputated fin at 0 dpa. (F) FK506-treated fin at 38 dpa. (G) Adult fin before amputation and cyclosporin A (CsA) treatment. (H) Amputated fin at 0 dpa. (I) CsA-treated fin at 38 dpa. (J) Graph of the fin-to-body ratios for DMSO-, FK506-, and CsA-treated fish. (K) Percentage of calcineurin activity normalized to DMSO in DMSO-, FK506-, and CsA-treated regenerating fins at 7 dpa. (L) ISH of calcineurin catalytic subunit in 5 dpa regenerating fins. (L2) Magnification of regenerating rays in (L). (M) Cross-section of adult caudal fin shows strong expression of calcineurin in the blastema and weak expression in the epidermis. (N) Fold change in calcineurin activity normalized to unamputated fins at different time points during regenerative outgrowth of the caudal fin of wild-type fish. p values given only for significance values of 0.05 or less. In (C), (F), and (I), black arrowheads mark the amputation planes. Black lines show the extent of regenerative outgrowth, and red lines highlight the length of the entire caudal fin. Scale bars, 1 mm (A–I), 200 μm (L and L2), and 20 μm (M). Data are represented as mean ± SD from three or more experiments with six or more animals. |
Reprinted from Developmental Cell, 28, Kujawski, S., Lin, W., Kitte, F., Börmel, M., Fuchs, S., Arulmozhivarman, G., Vogt, S., Theil, D., Zhang, Y., Antos, C.L., Calcineurin regulates coordinated outgrowth of zebrafish regenerating fins, 573-87, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell