FIGURE

Fig. 4

ID

ZDB-FIG-140527-19

Publication

Chen et al., 2014 - Glycogen synthase kinase 3 inhibitors induce the canonical WNT/β-catenin pathway to suppress growth and self-renewal in embryonal rhabdomyosarcoma

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Fig. 4

BIO activates canonical WNT/β-catenin signaling and induces differentiation of ERMS cells in vivo. (A–D) H&E-stained sections (A and C) and immunohistochemistry for β-catenin (B and D) of ERMS-bearing fish after 7 d of drug treatment with BIO (300 nM) or DMSO (vehicle control). (Scale bar, 25 μM.) (E) Quantitative RT-PCR analysis. (F) Pie charts showing the relative percentage of myf5:GFP⁺/mylz2:mCherry TPCs (green), myf5:GFP⁺/mylz2:mCherry⁺ (yellow), and late-differentiating myf5:GFP/mylz2:mCherry⁺ cells (red) for three independent ERMS as assessed by FACS (n > 3 animals per experimental arm and ±SD). *Statistically significant differences between DMSO and BIO treatments (Student t test, P < 0.05). (G) Summary of EDU staining of DMSO- or BIO-treated tumors. Each error bar indicates SEM of three tumors from each treatment group. *P < 0.005, Student t test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA