ZFIN ID: ZDB-FIG-140515-18
van Impel et al., 2014 - Divergence of zebrafish and mouse lymphatic cell fate specification pathways. Development (Cambridge, England)   141(6):1228-1238 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 3

Lymphatic specification is not blocked in prox1ai278;prox1bSA0035 double mutants. (A-C) In all genotype combinations of a prox1ai278;prox1bSA0035 double heterozygous incross, including double heterozygotes (A), homozygous prox1b mutants (B) and homozygous double mutants (C), PLs are apparent at the horizontal myoseptum at 2 dpf (arrows). (D-F) As in double heterozygous embryos (D), the loss of both copies of prox1b (E) does not lead to lymphatic defects on the level of TD formation. In prox1ai278;prox1bSA0035 double homozygous embryos (F), mild TD defects are occasionally visible (arrows indicate the TD and asterisks highlight segments without TD). (G) Average number of TD-positive segments within the first ten segments above the yolk extension for the indicated genotypic classes of a prox1a+/-;prox1b+/- incross. The moderate reduction in TD length is statistically significant in the prox1a single mutants (Student’s t-test, *P=0.01) and the prox1a;prox1b double mutant embryos (**P=0.002) when compared with wild-type siblings. Error bars represent s.d. (H) Overview about the average TD length (TD) in ten scored segments, the corresponding s.d. and the number of scored embryos (n) in a prox1a+/-;prox1b+/- incross. n.s., not statistically significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Citrine prox1bsa35/+; prox1ai278/+; hu5333Tg; hu7135Tg standard conditions Long-pec lymphatic system IFL
Long-pec vascular lymphangioblast IFL
Long-pec vein IFL
Day 5 lymphatic system IFL
Day 5 thoracic duct IFL
Day 5 vein IFL
prox1bsa35/sa35; hu5333Tg; hu7135Tg standard conditions Long-pec lymphatic system IFL
Long-pec vascular lymphangioblast IFL
Long-pec vein IFL
Day 5 lymphatic system IFL
Day 5 thoracic duct IFL
Day 5 vein IFL
prox1bsa35/sa35; prox1ai278/i278; hu5333Tg; hu7135Tg standard conditions Long-pec lymphatic system IFL
Long-pec vascular lymphangioblast IFL
Long-pec vein IFL
Day 5 lymphatic system IFL
Day 5 thoracic duct IFL
Day 5 vein IFL
RFP prox1bsa35/+; prox1ai278/+; hu5333Tg; hu7135Tg standard conditions Long-pec intersegmental vessel artery IFL
Day 5 intersegmental vessel artery IFL
prox1bsa35/sa35; hu5333Tg; hu7135Tg standard conditions Long-pec intersegmental vessel artery IFL
Day 5 intersegmental vessel artery IFL
prox1bsa35/sa35; prox1ai278/i278; hu5333Tg; hu7135Tg standard conditions Long-pec intersegmental vessel artery IFL
Day 5 intersegmental vessel artery IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
prox1ai278/i278; hu5333Tg; hu7135Tg standard conditions Day 5 thoracic duct decreased length, abnormal
Day 5 trunk anterior region lacks all parts of type thoracic duct, abnormal
prox1bsa35/sa35; prox1ai278/i278; hu5333Tg; hu7135Tg standard conditions Day 5 thoracic duct decreased length, abnormal
Day 5 trunk anterior region lacks all parts of type thoracic duct, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development