Fig. 9

PP1β genes interact genetically with Mypt1.

(A–L) Representative embryos at bud stage (A, C, E, G, I, K) and 48 hpf (B, D, F, H, J, L) injected with 15 pg mypt1 (1-300) mRNA with 100 pg of GFP mRNA (A–B), 50 pg each of ppp1cba and ppp1cbb mRNA with 15 pg GFP mRNA (C–D), 15 pg mypt1 mRNA + 50 pg each of ppp1cba and ppp1cbb mRNA (E–F), 0.5 ng of mypt1 MO and 0.25 ng of the control MO (G–H), 0.5 ng control MO and 0.25 ng 2MO (I–J), 0.5 ng mypt1 MO and 0.25 ng 2MO (0.125 ng ppp1cba MO and 0.125 ng of ppp1cbb MO) (K–L). Quantification of the angle of body axis extension at bud stage (M) and 48 hpf (N). Error bars are standard error and a * indicates a statistically significant difference from control and a # indicates a statistically significant rescue compared to morpholino injected embryos. For statistical analysis 50 embryos were analyzed for each condition. Statistical significance was calculated using a one-factor ANOVA with Tukey post hoc analysis and is defined as p < 0.05.