FIGURE

Fig. 1

ID

ZDB-FIG-131213-24

Publication

Schumacher et al., 2013 - tal1 regulates the formation of intercellular junctions and the maintenance of identity in the endocardium

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Fig. 1

Endocardium fails to populate the atrium in tal1-deficient embryos. (A–F) Lateral views of wild-type (WT) (A, C, and E) and tal1-deficient hearts (B, D, and F) in live embryos at 36 hpf; atrial end of the heart tube to the left. Three-dimensional reconstructions of confocal stacks depict myocardial cells expressing Tg(myl7:H2A-mCherry) (red; A, B, E, and F) and endothelial cells, including the endocardium, expressing Tg(fli1a:negfp) (green; A–D). In wild-type embryos (A and C), the endocardium fully lines the myocardial tube, and extends past the atrium. In tal1-deficient embryos (B and D), the endocardium is clumped within the ventricle (n=151/158).

Expression Data

Genes:	EGFP mCherry
Fish:	sd12Tg; y7Tg (AB) sd12Tg; y7Tg + MO5-tal1 + MO6-tal1 (AB)
Knockdown Reagents:	MO5-tal1 MO6-tal1
Anatomical Terms:	atrial endocardium atrial myocardium ventricular endocardium ventricular myocardium
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sd12Tg; y7Tg + MO5-tal1 + MO6-tal1 (AB)
Knockdown Reagents:	MO5-tal1 MO6-tal1
Observed In:	atrium endocardium morphogenesis ventricular endocardium
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 383(2), Schumacher, J.A., Bloomekatz, J., Garavito-Aguilar, Z.V., and Yelon, D., tal1 regulates the formation of intercellular junctions and the maintenance of identity in the endocardium, 214-226, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.