header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-131211-45
Kwon et al., 2013 - The parallel growth of motoneuron axons with the dorsal aorta depends on Vegfc/Vegfr3 signaling in zebrafish. Development (Cambridge, England)   140(19):4081-4090 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Day 4 to Day 5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Day 4 to Day 5

Fig. 6 Requirement of Vegfc and Vegfr3 for alignment of motoneuron axons and dorsal aorta. (A) 3D-rendered confocal stack images (lateral view) of Tg(fli1a:myr-mcherry);(mnx1:gfp)ml2 embryos treated with control DMSO (Ctrl, upper panels), with maz51 (middle panels) or with ki8751 (lower panels) at 4 dpf. Left panels, merged images of mCherry and GFP; right panels, GFP images. White arrows indicate the motoneurons beneath the DA. Arrowheads indicate the reduced growth of motoneuron axons beneath the DA. Yellow arrows indicate the slight reduction of growth of motoneuron axon beneath the DA. (B) 3D-rendered confocal stack images (lateral view) of Tg(fli1a:myr-mcherry);(mnx1:gfp)ml2 embryos treated with control MO (Ctrl, top panels), with vegfc MO (middle panels) or with vegfr3 MO (bottom panels). Left panels, merged images of mCherry and GFP; right panels, GFP images. Arrows indicate the motoneuron axons beneath the DA. Arrowheads indicate the reduced interaction of motoneurons and dorsal aorta. (C) Quantitative analyses of impairment of parallel growth (PG) of the embryos grouped as in B. The number (n) of the embryos examined for quantitative analyses is indicated at the top. (D) Immunohistochemical study of Tg(fli1a:egfp)y1 embryos (top) and vegfr3hu4602/wt(expando mutant);Tg(fli1a:egfp)y1 embryos using anti-znp1 antibody. Arrowheads and arrows indicate the presence or absence of axons beneath the dorsal aorta, respectively. Note that the TD is absent in the expando mutant. Scale bar: 25 μm. DA, dorsal aorta; TD, thoracic duct.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP flt4hu4602/+; s916Tg; y1Tg standard conditions Day 5 dorsal aorta IHC
ml2Tg; ncv1Tg control Day 4 motor neuron axon IFL
ml2Tg; ncv1Tg chemical treatment: pharmaceutical Day 4 motor neuron axon IFL
ml2Tg; ncv1Tg chemical treatment: pharmaceutical Day 4 motor neuron axon IFL
ml2Tg; ncv1Tg + MO1-flt4 standard conditions Day 4 motor neuron axon IFL
ml2Tg; ncv1Tg + MO4-vegfc standard conditions Day 4 motor neuron axon IFL
y1Tg standard conditions Day 5 dorsal aorta IHC
Day 5 thoracic duct IHC
mCherry ml2Tg; ncv1Tg control Day 4 dorsal aorta IFL
ml2Tg; ncv1Tg chemical treatment: pharmaceutical Day 4 dorsal aorta IFL
ml2Tg; ncv1Tg chemical treatment: pharmaceutical Day 4 dorsal aorta IFL
ml2Tg; ncv1Tg + MO1-flt4 standard conditions Day 4 dorsal aorta IFL
ml2Tg; ncv1Tg + MO4-vegfc standard conditions Day 4 dorsal aorta IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
znp-1 IHC flt4hu4602/+; s916Tg; y1Tg standard conditions Day 5 motor neuron axon
IHC y1Tg standard conditions Day 5 motor neuron axon
Phenotype Details
Fish Conditions Stage Phenotype
flt4hu4602/+; s916Tg; y1Tg standard conditions Day 5 motor neuron axon guidance process quality, abnormal
Day 5 thoracic duct absent, abnormal
ml2Tg; ncv1Tg chemical treatment: pharmaceutical Day 4 motor neuron axon guidance process quality, abnormal
ml2Tg; ncv1Tg + MO1-flt4 standard conditions Day 4 motor neuron axon guidance process quality, abnormal
ml2Tg; ncv1Tg + MO4-vegfc standard conditions Day 4 motor neuron axon guidance process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development