ZFIN ID: ZDB-FIG-131009-13
Chang et al., 2013 - Compensatory regulation of Na+ absorption by Na+/H+ exchanger and Na+-Cl- cotransporter in zebrafish (Danio rerio). Frontiers in Zoology   10(1):46 Full text @ Front. Zool.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 2

Loss of HR cells by gcm2 knockdown increases NCC cell number and Na+ absorption ability. (A, B): Immunostaining images of NCC cells (arrow) in 4-dpf wild type larvae (WT) and gcm2 morphants. Scale bar = 100 μm. (C): Comparison of NCC cell density between wild type larvae (white bars) and gcm2 morphants (gray bars) at different developmental stages (n = 8). (D): Na+ influx in 4-dpf wild types, gcm2 morphants, and foxi3a/3b morphants (n = 6). (E): Whole body Na+ content in wild type larvae (white bars) and gcm2 morphants (gray bars) at different developmental stages (n = 6). Mean ± SD. *p < 0.05 (Student’s t-test).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
slc12a10.2 Ab1-slc12a10.2 AB standard conditions Day 4 NCC ionocyte IHC
Ab1-slc12a10.2 AB + MO5-gcm2 standard conditions Day 4 NCC ionocyte IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-foxi3b + MO4-foxi3a standard conditions Day 4 sodium ion import across plasma membrane decreased occurrence, abnormal
AB + MO5-gcm2 standard conditions Protruding-mouth NCC ionocyte increased amount, abnormal
Protruding-mouth sodium ion homeostasis disrupted, abnormal
Day 4 NCC ionocyte increased amount, abnormal
Day 4 sodium ion import across plasma membrane increased occurrence, abnormal
Day 5 sodium ion homeostasis disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in Zoology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Zool.