ZFIN ID: ZDB-FIG-130828-25
Gupta et al., 2013 - An Injury-Responsive Gata4 Program Shapes the Zebrafish Cardiac Ventricle. Current biology : CB   23(13):1221-7 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
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Fish:
Conditions:
Anatomical Terms:
Stage: Days 30-44
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Days 30-44

Fig. 2

Experimental Injuries Stimulate Ectopic Cortical Muscle Formation (A) cmlc2:CreER; priZm zebrafish were labeled at 2 dpf, assessed at 42 dpf, and indicate no signs of cortical cardiomyocytes on the ventral ventricular surface. (B) When this region of the ventricle was pierced with a fine needle at 30 dpf, a small cortical clone (outlined in white; Cor) was detected at 42 dpf at the injury site in nearly half of the animals. (C) Section indicating a cortical clone (arrow) at 42 dpf, in a ventricle that had been pierced at 30 dpf. Arrowheads indicate the single-cardiomyocyte-thick primordial layer. (D and E) 30 dpf priZm; gata4:EGFP ventricles were pierced as in (A)–(C), with β-actin2-expressing cells indicated in red. Uninjured animals had no gata4:EGFP+ cardiomyocytes on the ventral surface of the ventricle at 42 dpf (D). By contrast, approximately half of the animals pierced at 30 dpf displayed a focus of gata4:EGFP+ cortical muscle at the site of trauma (E, arrow). (F) Higher-magnification confocal projection of injury site in (E). (G and H) Partial genetic ablation of cardiac muscle was initiated by brief 4-HT treatment at 30 dpf and analyzed 12 days later. 4-HT-treated Cre() ventricles contained no gata4:EGFP+ surface cardiomyocytes (G). By contrast, the ventricular surfaces of 4-HT-treated Cre(+) animals contained large amounts of gata4:EGFP+ cortical muscle (H). (I) Section indicating that gata4:EGFP+ cortical cardiomyocytes (arrow) are located on the outside of the primordial layer (arrowheads). Scale bars represent 100 μm (A, B, and D–H), 50 μm (C), and 25 μm (I). See also Figure S2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
CFP pd10Tg; pd50Tg chemical treatment: drug Days 30-44 Not Detected compact layer of ventricle cardiac muscle cell IFL
Days 30-44 trabecular layer of ventricle cardiac muscle cell IFL
pd10Tg; pd50Tg resection: cardiac ventricle, chemical treatment: drug Days 30-44 compact layer of ventricle cardiac muscle cell IFL
Days 30-44 trabecular layer of ventricle cardiac muscle cell IFL
GFP ae1Tg; pd10Tg; pd36Tg chemical treatment: drug Days 30-44 compact layer of ventricle cardiac muscle cell IFL
Days 30-44 trabecular layer of ventricle cardiac muscle cell IFL
ae1Tg; pd36Tg chemical treatment: drug Days 30-44 trabecular layer of ventricle cardiac muscle cell IFL
ae1Tg; pd50Tg standard conditions Days 30-44 Not Detected compact layer of ventricle cardiac muscle cell IFL
Days 30-44 Not Detected trabecular layer of ventricle cardiac muscle cell IFL
ae1Tg; pd50Tg physical alteration: cardiac ventricle Days 30-44 compact layer of ventricle cardiac muscle cell IFL
RFP ae1Tg; pd10Tg; pd36Tg chemical treatment: drug Days 30-44 trabecular layer of ventricle cardiac muscle cell IFL
ae1Tg; pd36Tg chemical treatment: drug Days 30-44 trabecular layer of ventricle cardiac muscle cell IFL
ae1Tg; pd50Tg standard conditions Days 30-44 trabecular layer of ventricle cardiac muscle cell IFL
ae1Tg; pd50Tg physical alteration: cardiac ventricle Days 30-44 trabecular layer of ventricle cardiac muscle cell IFL
pd10Tg; pd50Tg chemical treatment: drug Days 30-44 Not Detected compact layer of ventricle cardiac muscle cell IFL
Days 30-44 trabecular layer of ventricle cardiac muscle cell IFL
YFP pd10Tg; pd50Tg chemical treatment: drug Days 30-44 Not Detected compact layer of ventricle cardiac muscle cell IFL
Days 30-44 trabecular layer of ventricle cardiac muscle cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ae1Tg; pd10Tg; pd36Tg chemical treatment: drug Days 30-44 cardiac ventricle damaged, abnormal
Days 30-44 compact layer of ventricle cardiac muscle cell mislocalised, abnormal
Days 30-44 ventricular cardiac muscle tissue morphogenesis premature, abnormal
ae1Tg; pd50Tg physical alteration: cardiac ventricle Days 30-44 cardiac ventricle damaged, abnormal
Days 30-44 compact layer of ventricle cardiac muscle cell mislocalised, abnormal
Days 30-44 ventricular cardiac muscle tissue morphogenesis premature, abnormal
pd10Tg; pd50Tg resection: cardiac ventricle, chemical treatment: drug Days 30-44 cardiac ventricle damaged, abnormal
Days 30-44 compact layer of ventricle cardiac muscle cell mislocalised, abnormal
Days 30-44 ventricular cardiac muscle tissue morphogenesis premature, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.