ZFIN ID: ZDB-FIG-130828-24
Gupta et al., 2013 - An Injury-Responsive Gata4 Program Shapes the Zebrafish Cardiac Ventricle. Current biology : CB   23(13):1221-7 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Days 30-44 to Days 45-89
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 45-89

Fig. 1

gata4 Marks and Traces Emerging Juvenile Cortical Cardiomyocytes and Regenerating Cardiomyocytes (A) The 30 dpf ventricle comprises an outer primordial layer of single cardiomyocyte thickness. By <6 weeks postfertilization (wpf), inner trabecular cardiomyocytes (Tr) (green) breach the primordial layer (Pr) to establish a cortical cardiomyocyte clone (Cor) at the base of the ventricle. Top: histology section from multicolor clonal analysis, indicating breaching (arrows) of the primordial layer (arrowheads) by a trabecular myofiber (green). (B) During maturation, the initial basal clone (green) expands while other clones emerge on the ventricular surface. (C) Bottom: cortical layer development is completed by adulthood, with a small number of clones contributing to the entire cortical layer. Top: the adult ventricle retains an architecture with three muscle types. (D–F) Tissue sections of 6 (D and E) and 8 (F) weeks postfertilization (wpf) ventricular portions, assessed for tcf21:DsRed2+ epicardial cells (red) and gata4:EGFP+ cardiomyocytes (green). At 6 wpf, a cluster of gata4:EGFP+ surface myocytes is visible at the base of the heart in an area of dense epicardial tissue (arrow in D). By 8 wpf, gata4:EGFP+ cortical myocytes are detected at the ventricular midpoint, with cortical gata4:EGFP expression strongest in cardiomyocytes closer to the apex (arrow) and weaker in basal myocytes (arrowhead) (n = 8–12). Tr, trabeculae; Oft, Outflow tract. (G) gata4:ERCreER; priZm2 animals were pulsed with 4-HT at 6 wpf, and ventricles were analyzed at 90 dpf. Image shows surface muscle from a ventricle in these experiments with arrows indicating large clones. (H) Percentage surface area (SA) occupied by each clone from experiments in (G) (96 total counted clones from nine ventricular halves). (I) Cartoon of lineage-tracing experiments to determine clonal contributions in regenerating gata4+ cardiomyocytes. (J) Section through a ventricle from an uninjured animal 10 days after 4-HT treatment, indicating no recombination in the absence of injury. (K) Section through a ventricular apex at 30 dpa, visualizing multiple colored cardiomyocyte clones in regenerated muscle. Over 15 colors can be distinguished in this 10 µm section of the regenerate. A maximum projection image from confocal slices is shown (n = 6). (L) Higher-magnification view of regenerate shown in (K). Arrows indicate three differently colored multicellular clones. Scale bars represent 100 μm (D–G) and 50 μm (J–L). See also Figure S1 and Movie S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
CFP pd39Tg; pd50Tg chemical treatment: drug Days 45-89 Not Detected trabecular layer of ventricle cardiac muscle cell IFL
Days 45-89 Not Detected ventricular epicardium IFL
pd39Tg; pd50Tg resection: cardiac ventricle, chemical treatment: drug Days 45-89 trabecular layer of ventricle cardiac muscle cell IFL
DsRed2 ae1Tg; pd37Tg standard conditions Days 30-44 ventricular epicardium IFL
Days 45-89 ventricular epicardium IFL
GFP ae1Tg; pd37Tg standard conditions Days 30-44 ventricular myocardium cardiac muscle cell IFL
Days 45-89 compact layer of ventricle cardiac muscle cell IFL
RFP pd39Tg; pd50Tg chemical treatment: drug Days 45-89 trabecular layer of ventricle cardiac muscle cell IFL
Days 45-89 ventricular epicardium IFL
pd39Tg; pd50Tg resection: cardiac ventricle, chemical treatment: drug Days 45-89 trabecular layer of ventricle cardiac muscle cell IFL
Days 45-89 ventricular epicardium IFL
YFP pd39Tg; pd50Tg chemical treatment: drug Days 45-89 Not Detected trabecular layer of ventricle cardiac muscle cell IFL
Days 45-89 Not Detected ventricular epicardium IFL
pd39Tg; pd50Tg resection: cardiac ventricle, chemical treatment: drug Days 45-89 trabecular layer of ventricle cardiac muscle cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pd39Tg; pd50Tg resection: cardiac ventricle, chemical treatment: drug Days 45-89 cardiac muscle tissue regeneration increased process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.