bnc2 mutants exhibited reduced expression of melanogenic and xanthogenic factors.
(A) Wild-type. (B) Homozygous bnc2 mutant. (C) Quantitative RT-PCR for csf1a, csf1b, and kitlga revealed significantly reduced transcript abundances in skins isolated from 8.5 SSL bnc2 mutants as compared to stage-matched, wild-type bnc2/+ siblings. Shown are means±SE. Values are derived from 3 replicate experiments each consisting of 3 biological replicates for each genotype (n = 9 larvae total per genotype). Scale bar: in (B) 3 mm for (A,B).