ZFIN ID: ZDB-FIG-130807-24
Hochgreb-Hägele et al., 2013 - Laminin beta1a controls distinct steps during the establishment of digestive organ laterality. Development (Cambridge, England)   140(13):2734-2745 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 6 lamb1a knock-down in the KV as well as global knock-down of flh and ntl randomized the direction of LPM migration without altering epithelial polarity or composition of the LPM. (A-F) Expression of Tg(hand2:EGFP) in the LPM of embryos injected with control (A) or lamb1a (B-D) MO into the KV at 3 hpf, or flh MO (E) or ntl MO (F) at the one-cell stage. Transverse sections, dorsal towards the top. Scale bar: 10 μm. Dashed lines outline the LPM. L, left; R, right. (A′-F′) The LPM and gut shown in A-D. Gut (yellow), left LPM (red), right LPM (blue), Tg(hand2:EGFP)-expressing cells (green). (G) Proportions of control, lamb1a, flh and ntl MO-injected embryos exhibiting different directions of LPM migration. Effect of lamb1a knockdown in the KV on LPM migration is statistically significant, when compared with control, flh and ntl morphants (*P<0.001). The distribution of LPM migration phenotype is similar in flh and ntl morphants (P=0.794), but significantly different in embryos injected with lamb1a MO in the KV or with the control MO.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP pd24Tg control Prim-15 posterior lateral plate mesoderm IHC
pd24Tg + MO1-tbxta standard conditions Prim-15 posterior lateral plate mesoderm IHC
pd24Tg + MO2-lamb1a standard conditions Prim-15 posterior lateral plate mesoderm IHC
pd24Tg + MO3-noto standard conditions Prim-15 posterior lateral plate mesoderm IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pd24Tg + MO1-tbxta standard conditions Prim-15 determination of digestive tract left/right asymmetry decreased process quality, abnormal
Prim-15 lateral mesoderm morphogenesis decreased process quality, abnormal
Prim-15 lateral plate mesoderm bilateral symmetry, abnormal
Prim-15 lateral plate mesoderm mislocalised, abnormal
pd24Tg + MO2-lamb1a standard conditions Prim-15 determination of digestive tract left/right asymmetry decreased process quality, abnormal
Prim-15 lateral mesoderm morphogenesis decreased process quality, abnormal
Prim-15 lateral plate mesoderm bilateral symmetry, abnormal
Prim-15 lateral plate mesoderm mislocalised, abnormal
pd24Tg + MO3-noto standard conditions Prim-15 determination of digestive tract left/right asymmetry decreased process quality, abnormal
Prim-15 lateral mesoderm morphogenesis decreased process quality, abnormal
Prim-15 lateral plate mesoderm bilateral symmetry, abnormal
Prim-15 lateral plate mesoderm mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development