ZFIN ID: ZDB-FIG-130802-29
Lancman et al., 2013 - Specification of hepatopancreas progenitors in zebrafish by hnf1ba and wnt2bb. Development (Cambridge, England)   140(13):2669-2679 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Protruding-mouth

Fig. 3 hnf1ba and wnt2bb cooperate to specify the hepatopancreas organ system after mid-somitogenesis. (A-F) Three-dimensional rendering of 80 hpf Tg(ptf1a:eGFP)jh1; Tg(lfabp:dsRed)gz2 (pancreas, green; liver, red) foregut endoderm in wild type (A), wnt2bbs404 mutant (B), hnf1bas430 mutant (C) and wnt2bbs404; hnf1bas430 double mutants (D-F) stained for cadherin (epithelial endoderm) and with monoclonal antibody 2F11 to mark extra hepatopancreas ducts (*) (dashed outline). Relative to wild type, the wnt2bbs404 mutant liver (L) and the hnf1bas430 mutant pancreas (P) are reduced in size. The wnt2bbs404; hnf1bas430 double mutant liver and pancreas (arrow) are more severely reduced, or lost. Double mutants can exhibit complete loss of the hepatopancreas system (E,F; white arrowheads). (G,H) Three-dimensional rendering of 80 hpf Tg(ptf1a:eGFP)jh1 (pancreas, green) foregut endoderm in wild-type (G) and wnt2bbs404; hnf1bas430 double mutants (H) stained for cadherin and Isl1 to mark the hepatopancreas mesenchyme (brackets) and islet (I). Double mutants lacking the entire hepatopancreas system still develop Isl1+ mesenchyme and islet (yellow arrowheads indicate the swimbladder). (I) Merged fluorescent/brightfield micrographs of 4 dpf Tg(ptf1a:eGFP)jh1; Tg(lfabp:dsRed)gz2 wnt2bbs404 mutant (top) and wnt2bbs404; hnf1bas430 double mutants (bottom) showing no obvious body phenotypes other than liver and pancreas agenesis (arrows). (J,K) Three-dimensional rendering of 80 hpf foregut endoderm, heat-shock-induced Tg(hsp70l:dkk1-GFP)w32; Tg(ptf1a:eGFP)jh1; Tg(lfabp:dsRed)gz2 and Tg(hsp70l:dkk1-GFP)w32; hnf1bas430; Tg(ptf1a:eGFP)jh1; Tg(lfabp:dsRed)gz2 stained for cadherin and with 2F11. Heat-shock-induced expression of dkk1-GFP in wild-type background does not lead to liver or pancreas agenesis (J), whereas in the hnf1bas430 mutant background, the hepatopancreas system is completely lost (K) (white arrow). The swimbladder (yellow arrowhead) is not lost in wnt2bbs404; hnf1bas430 double mutants or in heat-shock-induced Tg(hsp70l:dkk1-GFP)w32; hnf1bas430 mutants (D-F,H,K).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hnf1bas430/s430; gz15Tg; jh1Tg standard conditions Protruding-mouth hepaticobiliary system development disrupted, abnormal
Protruding-mouth liver hypoplastic, abnormal
Protruding-mouth pancreas decreased size, abnormal
Protruding-mouth pancreas development disrupted, abnormal
hnf1bas430/s430; gz15Tg; jh1Tg; w32Tg heat shock Protruding-mouth hepaticobiliary system development disrupted, abnormal
Protruding-mouth liver agenesis, abnormal
Protruding-mouth pancreas agenesis, abnormal
Protruding-mouth pancreas development disrupted, abnormal
hnf1bas430/s430; wnt2bbs404/s404; gz15Tg; jh1Tg standard conditions Protruding-mouth hepaticobiliary system development disrupted, abnormal
Protruding-mouth liver decreased size, abnormal
Protruding-mouth pancreas decreased size, abnormal
Protruding-mouth pancreas development disrupted, abnormal
wnt2bbs404/s404; gz15Tg; jh1Tg standard conditions Protruding-mouth liver decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development