ZFIN ID: ZDB-FIG-130802-28
Lancman et al., 2013 - Specification of hepatopancreas progenitors in zebrafish by hnf1ba and wnt2bb. Development (Cambridge, England)   140(13):2669-2679 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 2 Distinct roles for Hnf1ba in regulating β-cell numbers and ventral pancreas specification. (A) β-Cell nuclei numbers from 80 and 125 hpf wild-type and hnf1bas430 mutants, indicating that at either stage, hnf1bas430 mutants have significantly fewer β-cells. hnf1bas430 mutants (125 hpf) have significantly fewer β-cells than 80 hpf wild-type embryos. Error bars represent s.d. (B-G) Fluorescent confocal microscopy of Tg(ptf1a:eGFP)jh1 80 hpf wild type (B-D) and 125 hpf hnf1bas430 mutant (E-G) pancreas stained for insulin antibodies, SV2 antibodies and DAPI to mark β-cells (red), endocrine cells (white) and nuclei (blue), respectively. Three-dimensional rendering of red and green channels showing an hnf1bas430 mutant at 125 hpf with a larger pancreas (E) than wild-type (B). (C,F) Magnification (8×) of B and E (red and white channels only) to show mildly disorganized islet cells. (D,G) Z-focal plane of C and F (with all channels) demonstrating moderately reduced β-cell number in the hnf1bas430 mutant islet. For a more severe example, see supplementary material Fig. S2. (H-M) Three-dimensional rendering of 52 hpf Tg(ptf1a:eGFP)jh1 (pancreas, green) foregut endoderm in wild-type (H), hnf1bahi2169 mutants (I) and hnf1bas430 embryos (J) stained for Pdx1 to mark the duodenal intestine and cadherin to mark the endoderm epithelium. In contrast to hnf1bahi2169 (I), Pdx1 expression (arrowheads) in the intestine and dorsal pancreatic islet (I) is not lost in hnf1bas430 embryos (J). (K-M) Three-dimensional rendering of 80 hpf Tg(ptf1a:eGFP)jh1; Tg(lfabp:dsRed)gz2 (pancreas, green; liver, red) foregut endoderm in wild-type (K) hnf1bahi2169 (L) and hnf1bas430 embryos (M) stained for cadherin showing specific loss of ventral pancreas in hnf1bas430 hypomorphic mutants. Unlike the hnf1bahi2169 (L), liver (L) and swimbladder (SB) are not lost in the hnf1bas430 embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed gz15Tg; jh1Tg standard conditions Protruding-mouth liver IFL
EGFP gz15Tg; jh1Tg standard conditions Protruding-mouth pancreas IFL
hnf1bas430/s430; jh1Tg standard conditions Day 5 pancreas IFL
jh1Tg standard conditions Protruding-mouth pancreas IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-SV2 IHC hnf1bas430/s430; jh1Tg standard conditions Day 5 endocrine cell
IHC jh1Tg standard conditions Protruding-mouth endocrine cell
Ab2-ins IHC hnf1bas430/s430; jh1Tg standard conditions Day 5 pancreatic B cell
IHC jh1Tg standard conditions Protruding-mouth pancreatic B cell
Phenotype Details
Fish Conditions Stage Phenotype
hnf1bahi2169Tg/hi2169Tg; gz15Tg; jh1Tg standard conditions Protruding-mouth liver agenesis, abnormal
Protruding-mouth pancreas agenesis, abnormal
Protruding-mouth pancreas development disrupted, abnormal
Protruding-mouth swim bladder agenesis, abnormal
hnf1bahi2169Tg/hi2169Tg; jh1Tg standard conditions Long-pec pancreas agenesis, abnormal
Long-pec pancreas development disrupted, abnormal
hnf1bas430/s430; gz15Tg; jh1Tg standard conditions Protruding-mouth pancreas agenesis, abnormal
Protruding-mouth pancreas development disrupted, abnormal
hnf1bas430/s430; jh1Tg standard conditions Long-pec pancreas agenesis, abnormal
Long-pec pancreas development disrupted, abnormal
Protruding-mouth pancreatic B cell decreased amount, abnormal
Protruding-mouth type B pancreatic cell development disrupted, abnormal
Day 5 islet disorganized, abnormal
Day 5 pancreatic B cell decreased amount, abnormal
Day 5 type B pancreatic cell development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development