FIGURE

Fig. 4

ID
ZDB-FIG-130726-6
Publication
Easley-Neal et al., 2013 - Late Recruitment of Synapsin to Nascent Synapses Is Regulated by Cdk5
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Synapsin1, STVs, and PTVs Are Transported Independently of Each Other (A–C) Selected sequence of five frames, 1 min apart, from 30 min time-lapse movies showing differential transport of synapsin1-GFP and VAMP2-mKate2 (A), synapsin1-mKate2 and N-cadherin-GFP (B), and N-cadherin-GFP and VAMP2-mKate2 (C) in RB axons. Movies acquired between 24–26 hpf. Arrows and arrowheads highlight motile puncta that were positive for only one of the two fluorescent fusion proteins. A VAMP2-mKate punctum was initially paused (yellow arrow in A) and colocalized with a synapsin1-GFP punctum (white arrow), but then moved away (t = 3.0). Scale bars represent 10 μm. (D) Histogram showing the number of two color puncta versus single color puncta that are motile over a 30 min imaging period. Two color puncta are those labeled for both transport packet markers imaged in a given pair, single color puncta are those expressing only one of the two markers. Error bars represent SEM. p < 0.005.(E) Distribution of the number of each type of puncta (N-cadherin, VAMP2, synapsin) at each velocity. The maximum velocity measured for a synapsin1 puncta was 2.49- and 1.98-fold slower than the maximum velocity for an N-cadherin or a VAMP2 puncta, respectively. See also Figure S2 and Movie S2.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.