ZFIN ID: ZDB-FIG-130726-42
Melvin et al., 2013 - A morpholino-based screen to identify novel genes involved in craniofacial morphogenesis. Developmental dynamics : an official publication of the American Association of Anatomists   242(7):817-31 Full text @ Dev. Dyn.
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Day 5

Fig. S7 Analysis of Adap1 expression and function in the larval zebrafish.
A, lateral view of a 24hpf zebrafish embryo showing expression of adap1 in the branchial arches. A′, dorsolateral view of embryo in (A) showing the branchial arch expression. B, lateral view of a 48hpf zebrafish embryo showing continued expression of adap1 in the pharyngeal endoderm. B′, rostral view of embryo in (B) showing adap1 expression around the stomodeum. C, lateral view of 24hpf uninjected embryo. D, lateral view of 24hpf larva after injection with 10ng adap1 SPL1 MO showing delayed somitogenesis and severely shortened body axis and yolk extension. Few adap1 morphants survive to 5dpf for analysis. E, lateral view of 5dpf adap1 morphant showing collapse of the rostral aspect of the head and curvature of the body axis. F, ventral view of adap1 morphant skeleton showing no changes to the cartilage structure.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
zgc:92360 WT standard conditions Prim-5 pharyngeal arch 3-7 ISH
Long-pec pharynx ISH
Long-pec stomodeum ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-zgc:92360 standard conditions Prim-5 somitogenesis delayed, abnormal
Prim-5 whole organism anterior-posterior axis shortened, abnormal
Day 5 head anterior side collapsed, abnormal
Day 5 whole organism dead, abnormal
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.