Cell autonomous effect of Sec13 depletion.
Dextran-fluorescin-labelled donor blastomeres were transplanted into host blastulas as indicated. Dextran-fluorescin was detected by TSA amplification (green), cones were identified by zpr-1 labelling (red) and nuclei were stained with DAPI (blue). Examples of blastomere transplants from/to LonAB embryos at 4dpf (A–C). Arrowheads (A): apoptotic morphant cells. Dotted lines (B): photoreceptor cells derived from the Sec13 MO host. Arrowheads (C): transplanted control cells. Blastomere transplantation into albino wild-type embryos (D–H). Sec13 morphant transplants were fluorescin-positive (green) or identified by presence of melanosomes (black cell layer, arrow in G). Graphical depiction (D) of the average number of transplanted blastomeres in each clone (black dots). Phenotypic transplants scored for impact on photoreceptors and/or lamination or no effect shown as fractions of 100% with respect to the number of clones in each category (F). Layers including photoreceptors (bold font) showed more effect. Photoreceptors and lamination: light blue, lamination: medium blue, photoreceptors: dark blue, no effect: black. Examples of transplants (green and/or pigmented) as indicated (E,E2,G,H). Photoreceptors (Zpr-1) in red and nuclei in blue. Arrow: Sec13 derived RPE cells; arrowheads: defects in retinal lamination; brackets: no effect on lamination; polygons: no effect on photoreceptors; star: autofluorescence of outer segments. Scale bars: 20μm.
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