ZFIN ID: ZDB-FIG-130416-13
Schmidt et al., 2013 - Early stages of retinal development depend on Sec13 function. Biology Open   2(3):256-266 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 3 Cellular identities in embryonic Sec13 knock-down eyes (A-T)
Immunohistochemistry of medial transversal sections. The following antibodies were used to identify cell types: zpr-1 (cones), zpr-3 (rods), HuC/D (amacrine and ganglion cells), zn-5 (ganglion cells incl. axons) and zrf-1 (Mueller glia). Insets in D,I,N,S: optic nerve/axons of ganglion cells. INL: inner nuclear layer; IPL: inner plexiform layer; ONL: outer nuclear layer; OPL: outer plexiform layer. Scale bars: 20μm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC WT control Protruding-mouth - Day 4 amacrine cell
IHC Protruding-mouth - Day 4 retinal ganglion cell
IHC WT + MO2-sec13 standard conditions Protruding-mouth - Day 4 amacrine cell
IHC Protruding-mouth - Day 4 retinal ganglion cell
zn-5 IHC WT control Protruding-mouth - Day 4 retinal ganglion cell
IHC WT + MO2-sec13 standard conditions Protruding-mouth - Day 4 retinal ganglion cell
zpr-1 IHC WT control Protruding-mouth - Day 4 retinal cone cell
IHC WT + MO2-sec13 standard conditions Protruding-mouth - Day 4 retinal cone cell
zpr-3 IHC WT control Protruding-mouth - Day 4 retinal rod cell
IHC WT + MO2-sec13 standard conditions Protruding-mouth - Day 4 retinal rod cell
zrf-1 IHC WT control Protruding-mouth - Day 4 Muller cell
IHC WT + MO2-sec13 standard conditions Protruding-mouth - Day 4 Muller cell
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-sec13 standard conditions Protruding-mouth amacrine cell decreased amount, abnormal
WT + MO2-sec13 standard conditions Protruding-mouth Muller cell decreased amount, abnormal
WT + MO2-sec13 standard conditions Protruding-mouth retinal cone cell decreased amount, abnormal
WT + MO2-sec13 standard conditions Protruding-mouth retinal ganglion cell decreased amount, abnormal
WT + MO2-sec13 standard conditions Protruding-mouth retinal rod cell aplastic, abnormal
WT + MO2-sec13 standard conditions Day 4 amacrine cell decreased amount, abnormal
WT + MO2-sec13 standard conditions Day 4 Muller cell disoriented, abnormal
WT + MO2-sec13 standard conditions Day 4 retina disorganized, abnormal
WT + MO2-sec13 standard conditions Day 4 retina layer formation disrupted, abnormal
WT + MO2-sec13 standard conditions Day 4 retinal cone cell decreased amount, abnormal
WT + MO2-sec13 standard conditions Day 4 retinal ganglion cell decreased amount, abnormal
WT + MO2-sec13 standard conditions Day 4 retinal rod cell aplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open