ZFIN ID: ZDB-FIG-130411-11
Boglev et al., 2013 - Autophagy induction is a tor- and tp53-independent cell survival response in a zebrafish model of disrupted ribosome biogenesis. PLoS Genetics   9(2):e1002379 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Conditions:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 5

Fig. 5 (A–F) Transverse sections (200 μm) through the intestinal bulb region of untreated WT (A) and ttis450 (B) larvae at 120 hpf or larvae previously treated for 14 h with rapamycin and/or chloroquine (C–F) stained with rhodamine phalloidin to detect F-actin (red), Hoechst 33342 to detect DNA (blue) and the LC3B antibody to detect LC3II–containing autophagosomes (green puncta). (G) The numbers of autophagosomes are increased in chloroquine-treated WT and ttis450 larvae compared to the corresponding untreated larvae. Chloroquine-treated ttis450 larvae contain significantly more puncta than chloroquine-treated WT larvae and similar numbers to WT larvae treated with rapamycin and chloroquine. Rapamycin and chloroquine-treated ttis450 larvae contain significantly more puncta per IEC than the IECs in chloroquine-treated ttis450 larvae and chloroquine and rapamycin-treated WT larvae. Puncta were counted in 20 cells from 3 independent sections using Metamorph. (H) Representative Western blot analysis of whole cell lysates of WT and ttis450 larvae (96 hpf) previously treated for 14 h with rapamycin (10 μM) and/or chloroquine (2.5 μM) using antibodies to LC3B and Actin (loading control). (I) Graphical representation of the data shown in H and two independent analyses. The LC3II signals were quantitated by densitometry. ttis450 larvae treated with chloroquine contain more LC3II than their chloroquine-treated WT siblings and comparable levels to WT larvae treated with rapamycin and chloroquine. Data are represented as mean +/ SD, *p<0.05.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
map1lc3b Ab3-map1lc3b WT control Day 5 Not Detected intestinal bulb epithelium autophagosome IHC
Ab3-map1lc3b WT chemical treatment: drug Day 5 intestinal bulb epithelium autophagosome IHC
Ab3-map1lc3b WT chemical treatment: sirolimus, chemical treatment: drug Day 5 intestinal bulb epithelium autophagosome IHC
Ab3-map1lc3b pwp2hs450/s450 standard conditions Day 5 intestinal bulb epithelium autophagosome IHC
Ab3-map1lc3b pwp2hs450/s450 chemical treatment: drug Day 5 intestinal bulb epithelium autophagosome IHC
Ab3-map1lc3b pwp2hs450/s450 chemical treatment: sirolimus, chemical treatment: drug Day 5 intestinal bulb epithelium autophagosome IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pwp2hs450/s450 chemical treatment: drug Day 5 autophagy increased occurrence, abnormal
Day 5 intestinal epithelium autophagosome increased amount, abnormal
pwp2hs450/s450 chemical treatment: sirolimus, chemical treatment: drug Day 5 autophagy increased occurrence, abnormal
Day 5 intestinal epithelium autophagosome increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.