ZFIN ID: ZDB-FIG-130210-9
Yin et al., 2012 - Inhibition of the pim1 oncogene results in diminished visual function. PLoS One   7(12):e52177 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Days 7-13

Fig. 5

Cell types in Socs1, Socs3a and Stat3 morphant retinae. Socs1, Socs3a and Stat3 morphant retinae were labelled with rhodopsin for photoreceptors, gfap:GFP for Muller glia, Hu for inner retinal neurons and TUNEL staining for apoptotic cells.

Scale bar in the upper left panel represents 50 microns and is the same for all the panels.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nt11Tg standard conditions Days 7-13 lens IFL
nt11Tg + MO3-socs1a standard conditions Days 7-13 lens IFL
nt11Tg + MO3-stat3 standard conditions Days 7-13 lens IFL
nt11Tg + MO3-stat3 + MO5-socs3a standard conditions Days 7-13 lens IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC WT standard conditions Days 7-13 lens
IHC Days 7-13 retinal inner nuclear layer
IHC WT + MO3-socs1a standard conditions Days 7-13 retinal inner nuclear layer
IHC WT + MO3-stat3 standard conditions Days 7-13 lens
IHC Days 7-13 retinal inner nuclear layer
IHC WT + MO3-stat3 + MO5-socs3a standard conditions Days 7-13 retinal inner nuclear layer
Ab1-opn1mw1 IHC nt11Tg standard conditions Days 7-13 retinal photoreceptor layer retinal cone cell
IHC nt11Tg + MO3-socs1a standard conditions Days 7-13 retinal photoreceptor layer retinal cone cell
IHC nt11Tg + MO3-stat3 standard conditions Days 7-13 retinal photoreceptor layer retinal cone cell
IHC nt11Tg + MO3-stat3 + MO5-socs3a standard conditions Days 7-13 retinal photoreceptor layer retinal cone cell
Ab2-rho IHC WT standard conditions Days 7-13 retinal photoreceptor layer retinal rod cell
IHC WT + MO3-socs1a standard conditions Days 7-13 retinal photoreceptor layer retinal rod cell
IHC WT + MO3-stat3 standard conditions Days 7-13 retinal photoreceptor layer retinal rod cell
IHC WT + MO3-stat3 + MO5-socs3a standard conditions Days 7-13 retinal photoreceptor layer retinal rod cell
Ab4-pcna IHC WT standard conditions Days 7-13 ciliary marginal zone
IHC WT + MO3-socs1a standard conditions Days 7-13 ciliary marginal zone
IHC WT + MO3-stat3 standard conditions Days 7-13 ciliary marginal zone
IHC WT + MO3-stat3 + MO5-socs3a standard conditions Days 7-13 ciliary marginal zone
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-socs1a standard conditions Days 7-13 eye apoptotic, abnormal
Days 7-13 eye decreased size, abnormal
WT + MO3-stat3 standard conditions Days 7-13 eye apoptotic, abnormal
Days 7-13 eye decreased size, abnormal
WT + MO3-stat3 + MO5-socs3a standard conditions Days 7-13 eye apoptotic, abnormal
Days 7-13 eye decreased size, abnormal
nt11Tg + MO3-socs1a standard conditions Days 7-13 eye decreased size, abnormal
nt11Tg + MO3-stat3 standard conditions Days 7-13 eye decreased size, abnormal
Days 7-13 retinal photoreceptor layer retinal cone cell decreased amount, abnormal
nt11Tg + MO3-stat3 + MO5-socs3a standard conditions Days 7-13 eye decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One