|ZFIN ID: ZDB-FIG-130129-18|
Fig. 2 Mpi knockdown decreases full-length LLO and mannose-6-phosphate levels in zebrafish. (A) Full-length LLO (G3M9) levels are decreased by Mpi knockdown, as seen on FACE analysis. (B) G3M9 LLO (normalized to number of fish) and mannose-6-phosphate (M6P) levels (normalized to 100 microgram protein) in lysates from 4 dpf zebrafish larvae. Values from larvae injected with the standard control morpholino were set as 100%. Average from three experiments. (C) N-glycans are decreased to 53% in mpi morphants on FACE analysis as compared with standard controls (n=2 experiments).
|AB + MO1-mpi||standard conditions||Day 4||dolichol-linked oligosaccharide biosynthetic process disrupted, abnormal|
|Day 4||oligosaccharide-lipid intermediate biosynthetic process disrupted, abnormal|
|Acknowledgments:||ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.|