ZFIN ID: ZDB-FIG-130117-42
Windner et al., 2012 - Fss/Tbx6 is required for central dermomyotome cell fate in zebrafish. Biology Open   1(8):806-814 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 3 Premature dermomyotome progenitor differentiation disrupts myotome patterning.

(A,B) Cross-sections and graphs showing dorsal-ventral distribution of dermomyotome (average of Pax7+ cells ± s.e.m.) and % differentiating dermomyotome (Pax7+/Mef2+) in wild type sibling and fss/tbx6 mutant (green, Pax7; red, Mef2; blue, Hoechst 33258). (C–L) Myotome morphology in wild type siblings (D–H) and fss/tbx6 (I–M) at the 24h stage. (C,H) Tracing of fast (white) and slow (red) muscle fibres on representative cross-sections. In fss/tbx6 mutants, the layer of slow fibres is disrupted and large fast muscle fibres are found lateral to the slow fibres in the central myotome. (D,E,I,J) Whole mounts (posterior trunk) and cross-sections labeled with F59 (red, labels slow myosin heavy chain at the 24h stage), Mef2 (aqua) and MF20 (white). (F,G,K,L) β-catenin labeled cross-sections double-labeled for either Pax7 (green, F,G) or 4D9 (red, engrailed, G,L); nuclei labeled with Hoechst. Arrowheads indicate large diameter, lateral fast fibres. N, notochord. Scale bars: 50 μm (A,C–E,G), 25 μm (F). Corresponding images of fss are to the same scale.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC WT standard conditions Prim-5 fast muscle cell
IHC tbx6te314a/te314a standard conditions Prim-5 fast muscle cell
IHC tbx6ti1/ti1 standard conditions Prim-5 fast muscle cell
Ab-MF20 IHC WT standard conditions Prim-5 muscle cell
IHC tbx6te314a/te314a standard conditions Prim-5 muscle cell
IHC tbx6ti1/ti1 standard conditions Prim-5 muscle cell
Ab-Pax7 IHC WT standard conditions Prim-5 dermomyotome
IHC tbx6te314a/te314a standard conditions Prim-5 dermomyotome
IHC tbx6ti1/ti1 standard conditions Prim-5 dermomyotome
Ab1-eng IHC WT standard conditions Prim-5 fast muscle cell
IHC Prim-5 slow muscle cell
IHC tbx6te314a/te314a standard conditions Prim-5 fast muscle cell
IHC Prim-5 slow muscle cell
IHC tbx6ti1/ti1 standard conditions Prim-5 fast muscle cell
IHC Prim-5 slow muscle cell
Ab1-mef2 IHC WT standard conditions Prim-5 dermomyotome
IHC tbx6te314a/te314a standard conditions Prim-5 dermomyotome
IHC tbx6ti1/ti1 standard conditions Prim-5 dermomyotome
Phenotype Details
Fish Conditions Stage Phenotype
tbx6te314a/te314a standard conditions Prim-5 myotome development process quality, abnormal
tbx6ti1/ti1 standard conditions Prim-5 myotome development process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open