ZFIN ID: ZDB-FIG-130109-21
Lai et al., 2012 - Autotaxin/Lpar3 signaling regulates Kupffer's vesicle formation and left-right asymmetry in zebrafish. Development (Cambridge, England)   139(23):4439-4448 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 5-9 somites to 10-13 somites

Fig. 3 Kupffer′s vesicle formation is disturbed in both lpar3 and atx morphants. (A-J,N) Tg(sox17:dsRed) zebrafish embryos were injected with MO and the integrity of KV was examined under differential interference contrast (A-E) or epifluorescence (F-J) microscopy. In contrast to Std control MO-injected embryos (A,F), deformed (B,G,D,I) and/or reduced KVs (C,H,E,J) were observed in both lpar3 and atx morphants (open arrowheads). In addition, dispersed dsRed-positive cells (arrowheads) were also observed around KV in both lpar3 and atx morphants. To quantify KV defects, Tg(sox17:dsRed) embryos were used to repeat the above experiments and analyses are shown in N. (K-M,O) Embryos injected with the indicated MO were subjected to WISH against charon (cha, ventral view, bottom right). Arrowheads point to KVs. Magnified KV insets are shown at the lower right of each panel; embryo midlines are indicated by dashed lines. WISH against charon also revealed similar deformed/reduced pattern in lpar3 (L) and atx (M) morphants compared with Std control MO-injected embryos (K). Quantitative analyses are shown in O.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s870Tg + MO2-lpar3 standard conditions 5-9 somites - 10-13 somites Kupffer's vesicle deformed, abnormal
s870Tg + MO5-enpp2 standard conditions 5-9 somites - 10-13 somites Kupffer's vesicle deformed, abnormal
s903Tg + MO2-lpar3 standard conditions 5-9 somites - 10-13 somites Kupffer's vesicle decreased size, abnormal
5-9 somites - 10-13 somites Kupffer's vesicle deformed, abnormal
5-9 somites - 10-13 somites Kupffer's vesicle cell mislocalised, abnormal
s903Tg + MO5-enpp2 standard conditions 5-9 somites - 10-13 somites Kupffer's vesicle decreased size, abnormal
5-9 somites - 10-13 somites Kupffer's vesicle deformed, abnormal
5-9 somites - 10-13 somites Kupffer's vesicle cell mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development