ZFIN ID: ZDB-FIG-121030-12
Martins Feitosa et al., 2012 - Hemicentin 2 and Fibulin 1 are required for epidermal-dermal junction formation and fin mesenchymal cell migration during zebrafish development. Developmental Biology   369(2):235-248 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 3 hmcn2/fbln1 double morphants display strongly compromised epidermal–dermal junction formation in the trunk, but not in the fin fold. (A, B) Transverse sections through trunk at 28 hpf, after anti-Laminin immunolabeling of BMs in green, Phalloidin-labeling of somitic muscle in red, and DAPI labeling of nuclei in blue. The double morphant (B) displays Laminin labeling at both sides of the blister, indicating that both epidermis and somites are properly attached to their respective basement membranes, and that blistering occurs within the dermal space in between. (C–K) Transmission electron micrographs of transverse sections through trunk (C–I) or dorsal tail fin (J, K) at stages indicated in upper right corners (for magnification, see scale bars). (C) Double morphant at low magnification, with most of trunk blister filled with amorphic material (indicated by arrows) at 55 hpf. (D, E) At 30 hpf, wild-type embryo (D) displays the first collagen fibers (arrow) underneath the yet indistinct BM, whereas much fewer fibers are present in the double morphant, with a fluid-filled blister underneath (E). Thinner and short fibers possibly integrated into and running perpendicular to the BM are visible in the double morphant (E; arrow), but more difficult to see in the wild-type (D, F), most likely due to the more compact organization of the tissue. It is tempting to speculate that they are the equivalent of the cross fibers of the fin fold (see Fig. 5), and involved in the organization of the ECM across the dermal space. (F, G) At 55 hpf, the collagenous network underneath the BM of the wild-type embryo consists of approximately ten layers organized in a plywood-like fashion (F; arrow), whereas the double morphant embryo displays a rupture within the second and third collagen layer (G; arrow). The shown specimen is one of the rare cases in which the blister seems to remain fluid-filled (see below). Arrowheads point to BMs. (H, I) In regions in which the architecture of the wild-type embryo requires a wider dermal space, such as at the base of the fins, it is filled with amorphic material underneath the sublaminal collagen fibers (H). In double morphants (I), most trunk blisters are filled with similar amorphic material, intermingled by disorganized and not properly anchored collagen fibers (arrows). (J, K) In the fin fold of wild-type embryos (J), collagenous actinotrichia are attached to the BM (indicated by arrowhead). In double morphant (K), actinotrichia and BM (indicated by arrowhead) appear unaffected, with rare and locally restricted detachments (indicated by arrow) in medio-basal regions of the fin. Abbreviations: act, actinotrichia; cf, cross fibers; dMO, double morphant; ep, epidermal cell; m, melanocyte.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-lama1 IHC WT standard conditions Prim-5 trunk basement membrane
IHC WT + MO1-fbln1 + MO1-hmcn2 standard conditions Prim-5 trunk basement membrane
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-fbln1 + MO1-hmcn2 standard conditions Prim-5 dermis separated from epidermis, abnormal
Prim-5 skin morphogenesis decreased process quality, abnormal
Prim-5 trunk integument blistered, abnormal
Prim-15 collagen fibril organization decreased process quality, abnormal
Prim-15 dermis fibrillar collagen trimer decreased amount, abnormal
Long-pec caudal fin actinotrichium attached to caudal fin basement membrane, normal
Long-pec collagen fibril organization decreased process quality, abnormal
Long-pec dermis separated from epidermis, abnormal
Long-pec dermis fibrillar collagen trimer disorganized, abnormal
Long-pec skin morphogenesis decreased process quality, abnormal
Long-pec trunk dermis malformed, abnormal
Long-pec trunk integument blistered, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 369(2), Martins Feitosa, N., Zhang, J., Carney, T.J., Metzger, M., Korzh, V., Bloch, W., and Hammerschmidt, M., Hemicentin 2 and Fibulin 1 are required for epidermal-dermal junction formation and fin mesenchymal cell migration during zebrafish development, 235-248, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.