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ZFIN ID: ZDB-FIG-120814-41
Izumi et al., 2012 - Fbxw7 controls angiogenesis by regulating endothelial notch activity. PLoS One   7(7):e41116 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 5 Impaired ISV growth after knockdown of zebrafish fbxw7 expression.

Following injection with control morpholino (control MO) or fbxw7 transcription blocking morpholino (fbxw7 ATG MO), the fluorescent vasculature of Tg(kdrl:EGFP)s843 transgenic embryos was examined by live microscopy. Outgrowth of ISVs from the dorsal aorta (DA) and formation of the DLAV (as indicated in the left image) were impaired in fbxw7 morphant embryos (arrowheads) at 32 hpf (A). Scale bar is 70 μm. Protein extracts from control and fbxw7 ATG MO embryos at 24 hpf and 32 hpf were analyzed by Western blotting (B). Note transient reduction of the Fbxw7 band (asterisk). Tubulin was used as loading control. Quantification of the number of ECs per ISV in control MO and fbxw7 ATG MO injected embryos at 32 hpf (based on 3 independent experiments) (C). Proliferation of endothelial cells at 32 hpf in ISVs of Tg(fli1a:nEGFP)y7 x Tg(kdrl:HsHRAS-mCherry)s896 double transgenic embryos injected with control MO or Fbxw7 ATG MO (D). Error bars indicate SEM. P value (*) in (C) is <0.05.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fbxw7 Ab1-fbxw7 WT control Prim-5 whole organism WB
Ab1-fbxw7 Prim-15 whole organism WB
Ab1-fbxw7 WT + MO2-fbxw7 standard conditions Prim-5 whole organism WB
Ab1-fbxw7 Prim-15 whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s843Tg + MO2-fbxw7 standard conditions Prim-15 angiogenesis disrupted, abnormal
Prim-15 intersegmental vessel decreased length, abnormal
Prim-15 whole organism lacks all parts of type dorsal longitudinal anastomotic vessel, abnormal
s896Tg; y7Tg + MO2-fbxw7 standard conditions Prim-15 angiogenesis disrupted, abnormal
Prim-15 intersegmental vessel endothelial cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One