FIGURE

Fig. S1

ID
ZDB-FIG-120720-49
Publication
Okuda et al., 2012 - lyve1 expression reveals novel lymphatic vessels and new mechanisms for lymphatic vessel development in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. S1

The lyve1 promoter marks regions of endogenous lyve1 expression and labelled trunk lymphatics develop in a pattern known to occur in the emergence of the thoracic duct. (A-L) lyve1 probe (A-D), gfp probe (E-H) and lyve1 (BM Purple) gfp (Fast Red) probes (I-L) applied to lyve1:EGFP embryos at 1 dpf (A,B,E,F,I,J) and 2 dpf (C,D,G,H,K,L) (A,C,E,G,I,K, lateral view; B,D,F,H,J,L dorsal view of anterior region). Black arrows indicate areas where gfp expression does not match endogenous lyve1 expression. (M-M′′) Lateral image of a lyve1:DsRed2;SAGFF27C;UAS:YFP transgenic at 5 dpf showing SAGFF27C;UAS:YFP expression (M), lyve1:DsRed2 (M′) and overlapping lyve1:DsRed2;SAGFF27C;UAS:YFP expression in the trunk lymphatic vessels (M′′). (N-N′′) Lateral image of a lyve1:EGFP;lyve1:DsRed2 transgenic at 5 dpf showing lyve1:EGFP expression (N), lyve1:DsRed2 (N′) and overlapping lyve1:EGFP;lyve1:DsRed2 expression in the trunk lymphatic vessels and PCV (N′′). (O-Q) Lateral images of the developing trunk lymphatics in the lyve1:EGFP transgenic at 1.3 dpf (O), 2 dpf (P) and 3 dpf (Q). (R,R′) Lateral image of the developed trunk lymphatics in lyve1:EGFP transgenic at 6 dpf (R). Microangiography confirms that these vessels contain no blood-flow (R′). DA, dorsal aorta; DLLV, dorsal longitudinal lymphatic vessel; ISLV, intersegmental lymphatic vessel; ISV, intersegmental vessel; LSS, lymphatic secondary sprout; PCV, posterior cardinal vein; PL, parachordal lymphangioblasts; TD, thoracic duct; vISV, venous intersegmental vessel. Scale bar: 100 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development