Okuda, K.S., Astin, J.W., Misa, J.P., Flores, M.V., Crosier, K.E., and Crosier, P.S. (2012) lyve1 expression reveals novel lymphatic vessels and new mechanisms for lymphatic vessel development in zebrafish. Development (Cambridge, England). 139(13):2381-2391.
We have generated novel transgenic lines that brightly mark the lymphatic system of zebrafish using the lyve1 promoter. Facilitated by these new transgenic lines, we generated a map of zebrafish lymphatic development up to 15 days
post-fertilisation and discovered three previously uncharacterised lymphatic vessel networks: the facial lymphatics, the lateral
lymphatics and the intestinal lymphatics. We show that a facial lymphatic vessel, termed the lateral facial lymphatic, develops
through a novel developmental mechanism, which initially involves vessel growth through a single vascular sprout followed
by the recruitment of lymphangioblasts to the vascular tip. Unlike the lymphangioblasts that form the thoracic duct, the lymphangioblasts
that contribute to the lateral facial lymphatic vessel originate from a number of different blood vessels. Our work highlights
the additional complexity of lymphatic vessel development in the zebrafish that may increase its versatility as a model of