Fig. S4

Additional examples of the fate-mapping of SPC. (A,B) Primordium region of 18s embryo injected with Kaede mRNA, before (A) and just after (B) photoactivation of Kaede in a small circular region including putative SPCs. The main primordium is outlined with white dashed lines. (C,D) Low (C) and high (D) magnification of the same embryo at the prim-15 stage, showing red cells (white arrowhead) in the leading zone of the migrating primordium. No deposited red cells are observed between the region of activation (red arrowhead) and the primordium. (E,F) Low (E) and high (F) magnification of the same embryo at 48h, showing the presence of red cells (white arrowheads) in the last deposited neuromast and in the primordium that has reached the tail region. No deposited red cells are detected more anteriorly. (G,H) Primordium region of 18s embryo injected with Kaede mRNA, before (G) and just after (H) photoactivation of Kaede in a region containing all potential SPCs, including half of somite 1 to somite 5. The main body of the primordium is outlined with white dashed lines. (I,J) Low (I) and high (J) magnification of the same embryo at the prim-11 stage, showing red cells (white arrowhead) scattered in the leading zone of the migrating primordium. No deposited red cell can be observed between the region of activation and the primordium. (K,L) Low (K) and high (L) magnification view of the same embryo at 48h, showing a large contribution of red cells (white arrowheads) to the three posterior-most neuromasts. No deposited red cells are detected more anteriorly. nt, neural tube; op, otic placode; S, somite. Scale bars: 25 μm.