FIGURE

Fig. S4

ID

ZDB-FIG-120316-98

Publication

Trofka et al., 2012 - The Her7 node modulates the network topology of the zebrafish segmentation clock via sequestration of the Hes6 hub

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Fig. S4

Her dimer binding to putative E boxes upstream of her7. (A-F) All homodimers determined previously to bind DNA Her1 (A), Her11 (B), Her12 (C), Her15 (D) were examined to determine whether they bind 11 E-boxes in the 3.7 kb of genomic sequence upstream of her7. Hes6VN+Hes6VC (E) was examined to ascertain whether stabilization of the Hes6 homodimer by the Venus BiFC would allow DNA binding. We also tested Her7 (F) to see whether it can bind to any of the E-boxes as a presumptive homodimer. (G-I) The Her7+Hes6 (G), Her12+Hes6 (H) and Her15+Her6 (I) heterodimers were tested for binding to the E-boxes. The Espl probe was excluded from the Her12+Hes6 and Her15+Her6 gels because the signal was too strong relative to the other E-boxes tested. These gels are representative and when exposed to film for longer times, weaker bands can be observed, as summarized in the chart in Fig 3C. (J,K) We tested the ability of Her7 (J) and Hes6 (K) to bind to the N-boxes using EMSA. No specific binding is observed. (L) A table of the E-box and N-box sequences for each probe. The E-box or N-box is underlined or in bold. e5 and e6 have 2 E-boxes close enough that they could not be resolved into separate probes and were therefore left together.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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