FIGURE

Fig. 2

ID

ZDB-FIG-120315-74

Publication

Shen et al., 2012 - The cytokine macrophage migration inhibitory factor (MIF) acts as a neurotrophin in the developing inner ear of the zebrafish, Danio rerio

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Fig. 2

Expression of mif and mif-like transcripts in the brain, eye and ear during embryogenesis. (A) RT-PCR results of mif-like mRNA from whole embryos at 12, 26, 36, 54, 60, and 72 hpf. (B–U) Whole mount ISH with mif or mif-like probes. (B–E) Dorsal view of 12-hpf embryos; (F–I) Dorsal view of 14-hpf embryos; (J–M) Lateral view of 24-hpf embryos; (N, P) Lateral view of 36-hpf embryos; (O, Q) Lateral view of 48-hpf embryos; (R–U) Lateral view of 72-hpf larvae. (B, F, J, N, O, R) mif antisense probe; (C, G, K, S) mif sense probe; (D, H, L, P, Q, T) mif-like anti-sense probe; (E, I, M, U) mif-like sense probe. Insets in (O) and (Q) show transverse sections through the ear; the small arrow in inset (Q) indicates hair cells in the anterior macula. Arrowheads: brain; OptV: optic vesicle; OV: otic vesicle; white arrows with black outlines: pectoral fin bud. Scale bar: 100 μm for (B–U); 20 μm for insets.

Expression Data

Genes:	ddt mif
Fish:	WT
Anatomical Terms:	brain eye hair cell anterior macula inner ear epithelial cell lens midbrain otic vesicle pectoral fin bud pharyngeal arch 3-7 retina solid lens vesicle whole organism whole organism anterior region
Stage Range:	5-9 somites to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 363(1), Shen, Y.C., Thompson, D.L., Kuah, M.K., Wong, K.L., Wu, K.L., Linn, S.A., Jewett, E.M., Shu-Chien, A.C., and Barald, K.F., The cytokine macrophage migration inhibitory factor (MIF) acts as a neurotrophin in the developing inner ear of the zebrafish, Danio rerio, 84-94, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.