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ZFIN ID: ZDB-FIG-120216-63
Yoruk et al., 2012 - Ccm3 functions in a manner distinct from Ccm1 and Ccm2 in a zebrafish model of CCM vascular disease. Developmental Biology   362(2):121-131 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 2

Morpholino knockdown of ccm3a/b causes gross dilation and mispatterning of cranial vessels. (A) At 54 hpf, confocal z-stack projections of dorsal head vasculature showed enlargements in the prosencephalic arteries (PrA), mesencephalic veins (MsV), and anterior cerebral veins (AceV) in embryos injected with 0.75 ng ccm3a/b morpholino (> 90%, n = 62). (B) Morpholino knockdown of ccm3a/b results in greater number of protrusions evident in endothelial cells of the dilated cranial vasculature (yellow arrows). (C) MsVs of the wildtype and ccm3a/b morphant embryos imaged at higher magnification in kdrl:HsHRAS-mCherry; fli1:nlsEGFP transgenic background. (D) ccm3a/b morphant embryos have a significantly higher number of cell protrusions per endothelial cell compared to wildtype (p-value < 0.01, n refers to number of embryos analyzed, with 3–5 cells counted per embryo).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP s843Tg standard conditions Long-pec cranial vasculature IFL
s843Tg + MO1-pdcd10a,pdcd10b standard conditions Long-pec cranial vasculature IFL
s896Tg; y7Tg standard conditions Long-pec mesencephalic vein IFL
s896Tg; y7Tg + MO1-pdcd10a,pdcd10b standard conditions Long-pec mesencephalic vein IFL
mCherry s896Tg; y7Tg standard conditions Long-pec mesencephalic vein IFL
s896Tg; y7Tg + MO1-pdcd10a,pdcd10b standard conditions Long-pec mesencephalic vein IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s843Tg + MO1-pdcd10a,pdcd10b standard conditions Long-pec anterior cerebral vein dilated, abnormal
Long-pec anterior cerebral vein increased thickness, abnormal
Long-pec blood vessel endothelial cell cell projection increased amount, abnormal
Long-pec central artery hypoplastic, abnormal
Long-pec cranial vasculature spatial pattern, abnormal
Long-pec mesencephalic vein dilated, abnormal
Long-pec mesencephalic vein increased thickness, abnormal
Long-pec prosencephalic artery dilated, abnormal
Long-pec prosencephalic artery increased thickness, abnormal
s896Tg; y7Tg + MO1-pdcd10a,pdcd10b standard conditions Long-pec blood vessel endothelial cell cell projection increased amount, abnormal
Long-pec blood vessel endothelial cell cell projection increased length, abnormal
Long-pec mesencephalic vein dilated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 362(2), Yoruk, B., Gillers, B.S., Chi, N.C., and Scott, I.C., Ccm3 functions in a manner distinct from Ccm1 and Ccm2 in a zebrafish model of CCM vascular disease, 121-131, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.